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Amacr (BC015825) Mouse Tagged ORF Clone
CAT＃: MG205514
- TrueORF®
Amacr (tGFP-tagged) - Mouse alpha-methylacyl-CoA racemase (cDNA clone MGC:13903 IMAGE:3980103)
ORF Plasmid: DDK
"BC015825" in other vectors (4)
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CNY 2,280.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Macr1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205514 representing BC015825
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCTGGCGGACTTCGGCGCCGAGGTGGTGCGCGTGAACCGGCTGGGCTCCACGGGCGAGAATTTTC TGGCCCGAGGCAAGCGCTCGCTAGCGCTGGACCTGAAGCGCTCTCAGGGAGTCACGGTGTTGCGGCGCAT GTGCGCACGCGCGGACGTGTTGCTGGAGCCCTTCCGCTGCGGTGTCATGGAGAAACTCCAGCTTGGGCCA GAGACTCTACTGCAGGACAATCCAAAGCTCATCTATGCCAGGCTGAGCGGATTTGGCCAATCGGGAATTT TCTCCAAAGTAGCTGGCCATGACATCAACTATTTGGCTTTATCAGGCGTTCTGTCAAAGATTGGCAGAAG CGGTGAGAACCCCTACCCACCGCTGAATCTCCTGGCTGACTTTGGCGGTGGAGGCCTCATGTGCACACTG GGCATTGTGCTGGCTCTCTTTGAACGCACACGCTCTGGCCGAGGGCAGGTCATCGATTCAAGCATGGTGG AAGGGACTGCATACTTAAGTTCTTTCCTGTGGAAAACCCAGCCCATGGGTCTGTGGAAACAGCCTCGAGG ACAAAACATCTTAGATGGCGGTGCACCTTTCTACACAACCTACAAGACGGCAGACGGGGAGTTCATGGCT GTAGGTGCCATAGAACCCCAGTTCTATGCACTGCTGCTTAAAGGACTTGGACTCGAGTCTGAGGAACTCC CCTCCCAGATGAGCTCAGCAGATTGGCCAGAGATGAAGAAGAAATTTGCAGATGTGTTTGCAAAGAAGAC TAAGGCAGAATGGTGCCAGATCTTTGACGGGACAGATGCGTGTGTGACCCCAGTGCTGACGTTTGAGGAG GCCCTCCACCACCAGCACAACAGAGAACGGGCCTCCTTCATCACTGATGGGGAGCAGCTCCCGAGCCCCC GCCCTGCACCTCTGCTTTCCAGAACTCCTGCCGTCCCATCTGCCAAAAGGGACCCTTCTGTAGGGGAGCA CACCGTAGAAGTGCTTAGAGAGTATGGATTCAGTCAGGAAGAGATCCTTCAGCTGCACTCAGATAGAATC GTTGAAAGTGATAAGCTAAAAGCCAATCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205514 representing BC015825
Red=Cloning site Green=Tags(s) MVLADFGAEVVRVNRLGSTGENFLARGKRSLALDLKRSQGVTVLRRMCARADVLLEPFRCGVMEKLQLGP ETLLQDNPKLIYARLSGFGQSGIFSKVAGHDINYLALSGVLSKIGRSGENPYPPLNLLADFGGGGLMCTL GIVLALFERTRSGRGQVIDSSMVEGTAYLSSFLWKTQPMGLWKQPRGQNILDGGAPFYTTYKTADGEFMA VGAIEPQFYALLLKGLGLESEELPSQMSSADWPEMKKKFADVFAKKTKAEWCQIFDGTDACVTPVLTFEE ALHHQHNRERASFITDGEQLPSPRPAPLLSRTPAVPSAKRDPSVGEHTVEVLREYGFSQEEILQLHSDRI VESDKLKANL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC015825 |
| ORF Size | 1082 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC015825, AAH15825 |
| RefSeq Size | 1472 bp |
| RefSeq ORF | 1082 bp |
| Locus ID | 17117 |
| Gene Summary | Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (By similarity). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200765 | Amacr (untagged) - Mouse alpha-methylacyl-CoA racemase (cDNA clone MGC:13903 IMAGE:3980103), (10ug) |
CNY 3,656.00 |
|
| MR205514 | Amacr (Myc-DDK-tagged) - Mouse alpha-methylacyl-CoA racemase (cDNA clone MGC:13903 IMAGE:3980103) |
CNY 3,656.00 |
|
| MR205514L3 | Lenti ORF clone of Amacr (Myc-DDK-tagged) - Mouse alpha-methylacyl-CoA racemase (cDNA clone MGC:13903 IMAGE:3980103) |
CNY 3,990.00 |
|
| MR205514L4 | Lenti ORF clone of Amacr (mGFP-tagged) - Mouse alpha-methylacyl-CoA racemase (cDNA clone MGC:13903 IMAGE:3980103) |
CNY 3,990.00 |
