Rnf146 (BC050795) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2610509H23Rik; Iduna
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205486 representing BC050795 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATGGCCGGCTGTGGTGAAATTGATCACTCAATAAATATGCTTCCTACAAATAAGAAGGCAAATG AGTCCTGTTCCAATACTGCACCTTCTCTGACAGTTCCTGAATGTGCCATTTGTCTACAAACATGTGTTCA CCCTGTCAGTCTGCCCTGTAAGCATGTTTTCTGTTATCTGTGTGTAAAGGGTGCTTCATGGCTTGGGAAG CGATGTGCTCTTTGTCGACAAGAGATTCCTGAGGATTTTCTTGACAAGCCAACCTTGTTGTCACCAGAAG AACTTAAGGCTGCAAGCAGAGGAAATGGTGAATATGCGTGGTATTATGAAGGAAGGAATGGGTGGTGGCA GTATGATGAGCGCACTAGTCGGGAGCTAGAAGATGCTTTTTCCAAAGGTAAAAAGAACACGGAAATGTTA ATTGCTGGATTTCTGTATGTTGCTGATCTTGAAAACATGGTTCAATATAGGAGAAATGAACATGGACGTC GCAGGAAGATTAAGCGAGATATAATAGATATACCAAAGAAGGGAGTAGCTGGACTTAGGCTGGACTGTGA CACCAATACTGTAAATCTAGCAAGAGAGAGTTCTGCTGATGGTGCGGACAGTGGATCAGCACAGACTGGA GCTTCTGTTCAGCTTGCAGTGCCATCTTCTACAAGGCCCCTGACATCAGTTGATGGTCAGTTAACCAGCC CTGTAACACCATCCCCTGATGCAGGCATTTCTTTGGAAGACTCTTTTGCTCATTTACAACTCAGTGGAGA CAGCATAGCTGAACGGAGTCACAGAGGTGAAGGAGAAGAAGATCATGAATCGCCATCTTCTGGCAGAGTA CCAGATACCTCCGTTGAAGAAACAGAATCAGATGCCAGTAGTGATAGTGAGGATGCCCCTGTGGTAGTTG CACAGCACTCTTTGACCCAACAGAGACCTTTGGTTCCAAATGGAAACCAGACAGTAGCCGACCAGTCAGA CCGATCAGGAACTGACCGATCAGTTGCAGGGGGTGGGACCATGAGTGTCAATGTCAGATCCAGAAGGCCT GATGGGCAGTGCACAGTGACAGAGGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205486 representing BC050795 Red=Cloning site Green=Tags(s) MEMAGCGEIDHSINMLPTNKKANESCSNTAPSLTVPECAICLQTCVHPVSLPCKHVFCYLCVKGASWLGK RCALCRQEIPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEML IAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDTNTVNLARESSADGADSGSAQTG ASVQLAVPSSTRPLTSVDGQLTSPVTPSPDAGISLEDSFAHLQLSGDSIAERSHRGEGEEDHESPSSGRV PDTSVEETESDASSDSEDAPVVVAQHSLTQQRPLVPNGNQTVADQSDRSGTDRSVAGGGTMSVNVRSRRP DGQCTVTEV TRTRPLE - GFP Tag - V
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC050795
ORF Size	1077 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050795, AAH50795
RefSeq Size	2355 bp
RefSeq ORF	1079 bp
Locus ID	68031
Gene Summary	E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination (By similarity). May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation.[UniProtKB/Swiss-Prot Function]

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