Gdf1 (BC079555) Mouse Tagged ORF Clone

CAT#: MG205443

  • TrueORF®

Gdf1 (tGFP-tagged) - Mouse growth differentiation factor 1 (cDNA clone MGC:90720 IMAGE:30359238)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms UOG-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG205443 representing BC079555
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCCTGTCTGCCATCGTTTTTGCGACCACCTCCTCCTCCTGCTCTTGCTGCCCTCGACGACCCTGG
CCCCCGCGCCAGCATCCATGGGCCCCGCTGCCGCCCTGCTCCAGGTTCTTGGGCTTCCCGAAGCGCCCCG
GAGCGTCCCCACACACCGACCTGTGCCTCCTGTCATGTGGCGCCTATTCCGTCGCCGCGACCCCCAGGAG
GCCAGAGTGGGACGCCCTCTGCGGCCATGCCACGTGGAGGAACTAGGGGTCGCCGGAAACATTGTGCGCC
ACATCCCCGACAGCGGTCTGTCCTCCAGGCCCGCACAACCCGCCAGGACCTCGGGGCTGTGCCCCGAGTG
GACAGTCGTCTTTGACCTGTCGAATGTGGAGCCCACAGAGCGCCCAACACGCGCGCGCTTAGAGTTGCGG
CTGGAGGCTGAGAGTGAAGATACAGGGGGGTGGGAGCTAAGCGTGGCACTGTGGGCCGACGCAGAGCATC
CAGGGCCTGAGCTGCTGCGCGTGCCGGCGCCACCAGGGGTGCTCCTGCGCGCAGACCTACTGGGGACTGC
AGTAGCCGCCAACGCATCAGTGCCCTGTACTGTGCGCCTGGCGCTGTCACTGCACCCTGGGGCCACTGCA
GCCTGTGGGCGCCTGGCTGAGGCCTCCCTGCTGCTGGTGACGCTGGACCCACGCCTGTGTCCCTTGCCGC
GATTGCGGCGCCACACGGAGCCCAGGGTAGAAGTTGGTCCAGTGGGCACTTGTCGTACCCGACGGTTGCA
TGTGAGCTTCCGTGAGGTGGGCTGGCACCGTTGGGTGATCGCGCCGCGTGGCTTCCTAGCCAACTTCTGC
CAGGGCACGTGCGCACTACCCGAAACGCTGAGGGGACCCGGCGGGCCGCCTGCACTCAACCACGCTGTGC
TGCGCGCGCTCATGCACGCAGCTGCTCCCACCCCGGGTGCAGGCTCGCCCTGCTGCGTGCCAGAGCGTCT
ATCACCCATCTCCGTGCTCTTCTTCGACAATAGTGACAACGTGGTCCTGCGACACTACGAAGACATGGTG
GTGGATGAGTGTGGCTGCCGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG205443 representing BC079555
Red=Cloning site Green=Tags(s)

MLPVCHRFCDHLLLLLLLPSTTLAPAPASMGPAAALLQVLGLPEAPRSVPTHRPVPPVMWRLFRRRDPQE
ARVGRPLRPCHVEELGVAGNIVRHIPDSGLSSRPAQPARTSGLCPEWTVVFDLSNVEPTERPTRARLELR
LEAESEDTGGWELSVALWADAEHPGPELLRVPAPPGVLLRADLLGTAVAANASVPCTVRLALSLHPGATA
ACGRLAEASLLLVTLDPRLCPLPRLRRHTEPRVEVGPVGTCRTRRLHVSFREVGWHRWVIAPRGFLANFC
QGTCALPETLRGPGGPPALNHAVLRALMHAAAPTPGAGSPCCVPERLSPISVLFFDNSDNVVLRHYEDMV
VDECGCR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC079555
ORF Size 1073 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC079555, AAH79555
RefSeq Size 1652 bp
RefSeq ORF 1073 bp
Locus ID 14559
Gene Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. This protein is translated from a monocistronic mRNA early in development, and from a bicistronic mRNA in later stages that also encodes ceramide synthase 1. [provided by RefSeq, Jul 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...