Gdf1 (BC079555) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	UOG-1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205443 representing BC079555 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCCTGTCTGCCATCGTTTTTGCGACCACCTCCTCCTCCTGCTCTTGCTGCCCTCGACGACCCTGG CCCCCGCGCCAGCATCCATGGGCCCCGCTGCCGCCCTGCTCCAGGTTCTTGGGCTTCCCGAAGCGCCCCG GAGCGTCCCCACACACCGACCTGTGCCTCCTGTCATGTGGCGCCTATTCCGTCGCCGCGACCCCCAGGAG GCCAGAGTGGGACGCCCTCTGCGGCCATGCCACGTGGAGGAACTAGGGGTCGCCGGAAACATTGTGCGCC ACATCCCCGACAGCGGTCTGTCCTCCAGGCCCGCACAACCCGCCAGGACCTCGGGGCTGTGCCCCGAGTG GACAGTCGTCTTTGACCTGTCGAATGTGGAGCCCACAGAGCGCCCAACACGCGCGCGCTTAGAGTTGCGG CTGGAGGCTGAGAGTGAAGATACAGGGGGGTGGGAGCTAAGCGTGGCACTGTGGGCCGACGCAGAGCATC CAGGGCCTGAGCTGCTGCGCGTGCCGGCGCCACCAGGGGTGCTCCTGCGCGCAGACCTACTGGGGACTGC AGTAGCCGCCAACGCATCAGTGCCCTGTACTGTGCGCCTGGCGCTGTCACTGCACCCTGGGGCCACTGCA GCCTGTGGGCGCCTGGCTGAGGCCTCCCTGCTGCTGGTGACGCTGGACCCACGCCTGTGTCCCTTGCCGC GATTGCGGCGCCACACGGAGCCCAGGGTAGAAGTTGGTCCAGTGGGCACTTGTCGTACCCGACGGTTGCA TGTGAGCTTCCGTGAGGTGGGCTGGCACCGTTGGGTGATCGCGCCGCGTGGCTTCCTAGCCAACTTCTGC CAGGGCACGTGCGCACTACCCGAAACGCTGAGGGGACCCGGCGGGCCGCCTGCACTCAACCACGCTGTGC TGCGCGCGCTCATGCACGCAGCTGCTCCCACCCCGGGTGCAGGCTCGCCCTGCTGCGTGCCAGAGCGTCT ATCACCCATCTCCGTGCTCTTCTTCGACAATAGTGACAACGTGGTCCTGCGACACTACGAAGACATGGTG GTGGATGAGTGTGGCTGCCGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205443 representing BC079555 Red=Cloning site Green=Tags(s) MLPVCHRFCDHLLLLLLLPSTTLAPAPASMGPAAALLQVLGLPEAPRSVPTHRPVPPVMWRLFRRRDPQE ARVGRPLRPCHVEELGVAGNIVRHIPDSGLSSRPAQPARTSGLCPEWTVVFDLSNVEPTERPTRARLELR LEAESEDTGGWELSVALWADAEHPGPELLRVPAPPGVLLRADLLGTAVAANASVPCTVRLALSLHPGATA ACGRLAEASLLLVTLDPRLCPLPRLRRHTEPRVEVGPVGTCRTRRLHVSFREVGWHRWVIAPRGFLANFC QGTCALPETLRGPGGPPALNHAVLRALMHAAAPTPGAGSPCCVPERLSPISVLFFDNSDNVVLRHYEDMV VDECGCR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC079555
ORF Size	1073 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC079555, AAH79555
RefSeq Size	1652 bp
RefSeq ORF	1073 bp
Locus ID	14559
Gene Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. This protein is translated from a monocistronic mRNA early in development, and from a bicistronic mRNA in later stages that also encodes ceramide synthase 1. [provided by RefSeq, Jul 2016]

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