Cxadr (BC016457) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	CAR, MCAR
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205349 representing BC016457 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGCCTACTGTGCTTCGTGCTCTTGTGCGGGATCGCGGATTTCACCAGTGGTTTGAGCATCACTA CACCCGAACAGAGGATCGAAAAAGCCAAAGGGGAAACTGCGTATCTACCATGCAAGTTTACTCTCAGTCC CGAAGACCAGGGACCACTGGACATTGAATGGCTGATATCCCCGTCTGATAACCAGATAGTGGATCAAGTG ATCATTTTGTATTCTGGAGACAAAATTTATGATAACTACTATCCGGATCTGAAAGGACGGGTACATTTTA CGAGTAACGATGTCAAGTCTGGCGACGCATCTATAAATGTGACCAACCTGCAGCTGTCGGACATTGGCAC TTACCAGTGCAAAGTGAAGAAAGCCCCTGGGGTTGCAAATAAGAAATTCCTGCTGACCGTTCTTGTTAAG CCTTCAGGTACAAGATGCTTCGTGGATGGATCGGAAGAGATTGGAAATGACTTCAAGCTAAAATGTGAAC CCAAGGAAGGCTCCCTTCCACTACAGTTTGAATGGCAGAAACTGTCGGACTCCCAGACAATGCCTACGCC ATGGCTGGCAGAAATGACGTCACCAGTTATATCTGTGAAGAACGCCAGTTCTGAGTATTCTGGGACATAC AGCTGCACGGTTCAAAACAGAGTGGGCTCTGACCAGTGTATGCTGCGACTAGACGTTGTCCCACCCTCCA ACCGAGCCGGAACGATCGCGGGCGCCGTCATAGGGACGCTGCTGGCCCTTGTGCTCATCGGGGCCATCCT CTTCTGCTGTCACAGGAAACGCAGAGAAGAGAAGTACGAGAAGGAAGTTCATCATGATATCAGGGAAGAT GTGCCTCCTCCAAAGAGTCGGACATCCACTGCCAGGAGCTATATTGGCAGCAACCATTCCTCCCTGGGAT CCATGTCCCCCTCTAACATGGAGGGGTATTCCAAGACGCAGTATAACCAAGTCCCCAGTGAAGACTTTGA ACGTGCGCCTCAGAGCCCGACTCTGGCACCCGCTAAGTTCAAGTACGCTTACAAGACCGATGGCATTACA GTGGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205349 representing BC016457 Red=Cloning site Green=Tags(s) MARLLCFVLLCGIADFTSGLSITTPEQRIEKAKGETAYLPCKFTLSPEDQGPLDIEWLISPSDNQIVDQV IILYSGDKIYDNYYPDLKGRVHFTSNDVKSGDASINVTNLQLSDIGTYQCKVKKAPGVANKKFLLTVLVK PSGTRCFVDGSEEIGNDFKLKCEPKEGSLPLQFEWQKLSDSQTMPTPWLAEMTSPVISVKNASSEYSGTY SCTVQNRVGSDQCMLRLDVVPPSNRAGTIAGAVIGTLLALVLIGAILFCCHRKRREEKYEKEVHHDIRED VPPPKSRTSTARSYIGSNHSSLGSMSPSNMEGYSKTQYNQVPSEDFERAPQSPTLAPAKFKYAYKTDGIT VV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC016457
ORF Size	1058 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016457, AAH16457
RefSeq Size	1696 bp
RefSeq ORF	1058 bp
Locus ID	13052
Gene Summary	This gene encodes a protein that is part of the Cortical Thymocyte marker in Xenopus (CTX) subfamily within the immunoglobulin superfamily. Members of this subfamily, predominantly expressed on the surface of endothelial and epithelial cells, help establish cell polarity and provide a barrier function, regulating migration of immune cells. This protein, first identified as the receptor for adenovirus subgroup C and coxsakieviruses group B, is developmentally regulated and plays an important role in cardiac development. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

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