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Tfb1m (BC032930) Mouse Tagged ORF Clone
CAT＃: MG205191
- TrueORF®
Tfb1m (tGFP-tagged) - Mouse transcription factor B1, mitochondrial (cDNA clone MGC:41387 IMAGE:1382837)
ORF Plasmid: DDK
"BC032930" in other vectors (3)
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CNY 2,190.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC41387 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205191 representing BC032930
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCTCGGGGAAGCTCGGCACTTTCCGCCTTCCCCCGTTGCCCACCATTCGGGAGATCATTAAGT TGTTCGGACTGCGAGCGGTGAAACAGCTTTCCCAGAATTTCCTCCTGGACTTGAGGCTGACAGACAAGAT TGTAAGGAAAGCTGGCAGTCTAGCAGATGTTTATGTTTATGAAGTGGGTCCTGGGCCCGGGGGAATCACA AGATCCATCCTGAATGCCAACGTTGCTGAGCTTCTGGTGGTTGAAAAGGACACTCGCTTTATCCCAGGGT TACAGATGCTCTCTGATGCAGCACCTGGAAAACTCCGAATTGTTCATGGAGATGTGCTGACATACAAGAT AGAAAAGGCTTTTCCAGGCAACATTAGAAGACAGTGGGAAGATGATCCTCCAAATGTACACATTATTGGA AATCTACCTTTTAGTGTTTCAACTCCACTGATTATCAAGTGGCTTGAAAATATTTCTCTTAAAGATGGCC CTTTCGTTTATGGCAGAACTAAGATGACGTTAACGTTTCAAAAGGAAGTGGCTGAGAGACTTGTAGCCAC TACAGGAAGCAAACAGCACAGTCGCCTTTCCATTATGGCCCAGTACCTCTGCAATGTTGAACACCTCTTT ACAATCCCAGGAAAAGCTTTTGTTCCCAAACCAAAAGTTGATGTAGGAGTGGTGCACCTTACGCCCTTGA TAGAGCCCAAGATCAAGCAGCCATTCAAGCTGGTAGAAAAAGTTGTCCAGAATGCGTTTCAGTTTCGAAG GAAATATTGCCACCGAGGGCTTGGAATGTTATTCCCTGAAGCACAACGCCTCGAAAGCACTGGAAGGCTG TTACAGTTGGCAGACATTGATCCAACTCTCCGGCCCACCCACCTCTCGCTCATGCATTTTAAGAGTTTGT GTGATGTATACAGAAAAATGTGTGATGAAGACCCACAACTCTTTACTTATAACTTCAGAGAGGAGCTCAA GCAAAAGAAAAGTAAAGGCCAAGAAAAAGATGGTGACCCAGAAAGCTGCGGGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205191 representing BC032930
Red=Cloning site Green=Tags(s) MAASGKLGTFRLPPLPTIREIIKLFGLRAVKQLSQNFLLDLRLTDKIVRKAGSLADVYVYEVGPGPGGIT RSILNANVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTYKIEKAFPGNIRRQWEDDPPNVHIIG NLPFSVSTPLIIKWLENISLKDGPFVYGRTKMTLTFQKEVAERLVATTGSKQHSRLSIMAQYLCNVEHLF TIPGKAFVPKPKVDVGVVHLTPLIEPKIKQPFKLVEKVVQNAFQFRRKYCHRGLGMLFPEAQRLESTGRL LQLADIDPTLRPTHLSLMHFKSLCDVYRKMCDEDPQLFTYNFREELKQKKSKGQEKDGDPESCGF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC032930 |
| ORF Size | 1037 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC032930, AAH32930 |
| RefSeq Size | 1360 bp |
| RefSeq ORF | 1037 bp |
| Locus ID | 224481 |
| Gene Summary | S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR205191 | Tfb1m (Myc-DDK-tagged) - Mouse transcription factor B1, mitochondrial (cDNA clone MGC:41387 IMAGE:1382837) |
CNY 3,656.00 |
|
| MR205191L3 | Lenti ORF clone of Tfb1m (Myc-DDK-tagged) - Mouse transcription factor B1, mitochondrial (cDNA clone MGC:41387 IMAGE:1382837) |
CNY 3,900.00 |
|
| MR205191L4 | Lenti ORF clone of Tfb1m (mGFP-tagged) - Mouse transcription factor B1, mitochondrial (cDNA clone MGC:41387 IMAGE:1382837) |
CNY 3,900.00 |
