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Ubiad1 (BC015303) Mouse Tagged ORF Clone
CAT＃: MG204995
- TrueORF®
Ubiad1 (tGFP-tagged) - Mouse UbiA prenyltransferase domain containing 1 (cDNA clone MGC:19220 IMAGE:4241204)
ORF Plasmid: DDK
"BC015303" in other vectors (3)
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CNY 2,090.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1200002M06Rik; AI426463; AW320947; Tere1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204995 representing BC015303
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGTACAGGCCCCGGGGGAGAAGATTAATATCCTGGCAGGAGAGACAGCCAAGGTCGGGGACC CGCAGAAGAACGAATGGCCCGAGCAGGACAGGCTTCCCGAACGATCCTGGAGGCACAAGTGCGCCTCCTA CGTGTTGGCCCTGAGGCCCTGGAGCTTCAGTGCCTCACTCACCCCTGTGGCCCTGGGCAGTGCCTTGGCC TACAGGTCTCAGGGTGTCCTGGATCCCAGGCTGTTGTTGGGTTGTGCAGTGGCTGTCCTGGCTGTACACG GGGCCGGCAATTTGGTCAACACATACTATGACTTTTCCAAGGGCATTGACCACAAAAAGAGTGATGACAG AACTTTGGTGGACAGAATTCTGGAGCCCCAGGATGTTGTTCGATTTGGAGTCTTCCTCTACACCTTGGGC TGCGTCTGTGCTGCTTGCCTCTACTACCTGTCCGCTCTGAAATTGGAACACTTGGCTCTCATCTACTTCG GAGGCCTGTCTGGCTCCTTTCTCTACACAGGAGGAATTGGATTCAAGTATGTGGCCCTGGGAGACCTCGT CATCCTCATCACTTTCGGCCCGCTGGCTGTGATGTTTGCCTACGCTGTCCAGGTGGGATCCCTGGCCATC TTCCCTCTAATCTACGCCATCCCTCTGGCCCTCAGCACGGAGGCCATTCTCCATTCCAACAACACCAGGG ACATGGAATCTGACCGAGAGGCTGGCATCGTCACGCTGGCCATCCTCATTGGGCCCACCTTCTCCTATGT CCTCTATAACACACTGCTCTTTGTGCCCTACCTAATCTTTACCATCCTGGCCACGCACTGCAGCATCAGC CTGGCACTGCCCCTGCTCACCATCCCCATGGCCTTCTCCCTTGAGAGGCAGTTCCGCAGCCAGGCCTTCA ACAAGCTGCCCCAGAGGACAGCCAAGCTCAACCTCCTGCTGGGGCTTTTCTATGTCTTTGGCATCATCCT GGCACCAGCAGGCAGCCTGCCCAGACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204995 representing BC015303
Red=Cloning site Green=Tags(s) MAAVQAPGEKINILAGETAKVGDPQKNEWPEQDRLPERSWRHKCASYVLALRPWSFSASLTPVALGSALA YRSQGVLDPRLLLGCAVAVLAVHGAGNLVNTYYDFSKGIDHKKSDDRTLVDRILEPQDVVRFGVFLYTLG CVCAACLYYLSALKLEHLALIYFGGLSGSFLYTGGIGFKYVALGDLVILITFGPLAVMFAYAVQVGSLAI FPLIYAIPLALSTEAILHSNNTRDMESDREAGIVTLAILIGPTFSYVLYNTLLFVPYLIFTILATHCSIS LALPLLTIPMAFSLERQFRSQAFNKLPQRTAKLNLLLGLFYVFGIILAPAGSLPRL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC015303 |
| ORF Size | 1010 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC015303, AAH15303 |
| RefSeq Size | 2694 bp |
| RefSeq ORF | 1010 bp |
| Locus ID | 71707 |
| Gene Summary | Prenyltransferase that mediates the formation of menaquinone-4 (MK-4) and coenzyme Q10. MK-4 is a vitamin K2 isoform required for endothelial cell development. Mediates the conversion of phylloquinone (PK) into MK-4, probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. Also plays a role in cardiovascular development independently of MK-4 biosynthesis, by acting as a coenzyme Q10 biosynthetic enzyme: coenzyme Q10, also named ubiquinone, plays an important antioxidant role in the cardiovascular system. Mediates biosynthesis of coenzyme Q10 in the Golgi membrane, leading to protect cardiovascular tissues from NOS3/eNOS-dependent oxidative stress (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR204995 | Ubiad1 (Myc-DDK-tagged) - Mouse UbiA prenyltransferase domain containing 1 (cDNA clone MGC:19220 IMAGE:4241204) |
CNY 3,656.00 |
|
| MR204995L3 | Lenti ORF clone of Ubiad1 (Myc-DDK-tagged) - Mouse UbiA prenyltransferase domain containing 1 (cDNA clone MGC:19220 IMAGE:4241204) |
CNY 3,800.00 |
|
| MR204995L4 | Lenti ORF clone of Ubiad1 (mGFP-tagged) - Mouse UbiA prenyltransferase domain containing 1 (cDNA clone MGC:19220 IMAGE:4241204) |
CNY 3,800.00 |
