Suclg1 (NM_019879) Mouse Tagged ORF Clone
CAT#: MG204912
- TrueORF®
Suclg1 (tGFP-tagged) - Mouse succinate-CoA ligase, GDP-forming, alpha subunit (Suclg1)
ORF Plasmid: DDK
"NM_019879" in other vectors (4)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1500000I01Rik; Sucla1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204912 representing NM_019879
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTCCAGCAGCAGCGGCCTCGCCGCCGCCCGCCTGTTGTCGCGCACCTTCCTCCTGCAACAGAATG GGATACGACACGGGTCTTACACAGCCTCTCGGAAACATATCTATATTGATAAAAATACGAAGATTATTTG CCAGGGTTTCACAGGCAAACAGGGTACCTTTCACAGCCAGCAGGCTTTGGAGTACGGCACCAAACTCGTG GGAGGAACCACTCCAGGGAAGGGTGGCCAGAAGCACCTGGGCTTGCCCGTCTTTAATACTGTGAAGGAAG CCAAAGAGAAAACAGGAGCGACGGCTTCTGTCATCTATGTCCCTCCTCCTTTTGCTGCTGCTGCCATTAA TGAAGCAATCGACGCGGAGATTCCCTTGGTTGTGTGCATTACGGAAGGTATTCCGCAGCAGGATATGGTG CGGGTCAAGCACAGACTGACACGCCAGGGAACGACGAGGCTAATCGGGCCAAACTGCCCCGGCGTCATCA ACCCTGGAGAATGCAAAATCGGCATCATGCCTGGCCACATTCACAAGAAGGGAAGAATAGGTATCGTGTC CAGGTCCGGTACTCTGACTTACGAAGCAGTTCACCAAACAACCCAAGTCGGATTGGGGCAGTCCTTGTGT ATTGGCATTGGAGGTGACCCTTTTAATGGGACTGATTTTATTGATTGCCTTGAAGTCTTCCTGAATGATC CAGCCACAGAAGGCATCATACTGATTGGTGAAATTGGTGGTCACGCTGAAGAAAATGCTGCCGCGTTTCT GAAGGAGCATAACTCAGGTCCAAAGGCCAAGCCTGTAGTGTCCTTCATTGCTGGTATAACTGCTCCTCCT GGCAGAAGGATGGGCCATGCAGGGGCAATTATTGCTGGAGGAAAAGGTGGCGCTAAAGAGAAGATCTCTG CTCTTCAGAGTGCTGGGGTGGTTGTCAGCATGTCCCCCGCACAGCTGGGAACTACCATTTATAAGGAGTT TGAAAAGAGGAAGATGCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204912 representing NM_019879
Red=Cloning site Green=Tags(s) MVSSSSGLAAARLLSRTFLLQQNGIRHGSYTASRKHIYIDKNTKIICQGFTGKQGTFHSQQALEYGTKLV GGTTPGKGGQKHLGLPVFNTVKEAKEKTGATASVIYVPPPFAAAAINEAIDAEIPLVVCITEGIPQQDMV RVKHRLTRQGTTRLIGPNCPGVINPGECKIGIMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLC IGIGGDPFNGTDFIDCLEVFLNDPATEGIILIGEIGGHAEENAAAFLKEHNSGPKAKPVVSFIAGITAPP GRRMGHAGAIIAGGKGGAKEKISALQSAGVVVSMSPAQLGTTIYKEFEKRKML TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019879 |
ORF Size | 999 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019879.1, NP_063932.1 |
RefSeq Size | 1212 bp |
RefSeq ORF | 1041 bp |
Locus ID | 56451 |
UniProt ID | Q9WUM5 |
Gene Summary | Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201005 | Suclg1 (untagged) - Mouse succinate-CoA ligase, GDP-forming, alpha subunit (Suclg1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,656.00 |
|
MR204912 | Suclg1 (Myc-DDK-tagged) - Mouse succinate-CoA ligase, GDP-forming, alpha subunit (Suclg1), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
MR204912L3 | Lenti ORF clone of Suclg1 (Myc-DDK-tagged) - Mouse succinate-CoA ligase, GDP-forming, alpha subunit (Suclg1), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
|
MR204912L4 | Lenti ORF clone of Suclg1 (mGFP-tagged) - Mouse succinate-CoA ligase, GDP-forming, alpha subunit (Suclg1), nuclear gene encoding mitochondrial protein |
CNY 6,056.00 |