Alad (NM_008525) Mouse Tagged ORF Clone
CAT#: MG204863
- TrueORF®
Alad (tGFP-tagged) - Mouse aminolevulinate, delta-, dehydratase (Alad)
ORF Plasmid: DDK
"NM_008525" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ALADH; Lv |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204863 representing NM_008525
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCACCAGTCTGTTCTGCACAGCGGCTACTTTCACCCACTGCTTCGGAGCTGGCAGACTGCTGCCT CCACCGTCAGTGCCTCCAACCTCATCTATCCCATCTTTGTCACGGATGTTCCTGATGATGTCCAGCCTAT CGCCAGCCTCCCAGGAGTGGCCAGGTATGGCGTAAACCAGCTAGAAGAGATGCTGAGACCTCTGGTGGAA GCTGGCCTGCGCTGTGTCCTGATCTTTGGCGTCCCCAGCAGAGTTCCCAAGGATGAACAGGGCTCTGCAG CTGACTCTGAGGACTCCCCAACTATTGAGGCTGTCCGTCTGCTGAGGAAGACCTTCCCTTCCCTCCTAGT GGCCTGTGACGTCTGCTTGTGCCCCTACACCTCCCATGGCCACTGTGGCCTCCTGAGTGAAAATGGAGCA TTCCTAGCAGAGGAGAGCCGACAGCGGTTGGCAGAGGTGGCACTGGCCTATGCCAAGGCAGGCTGTCAGG TTGTAGCTCCGTCAGACATGATGGACGGACGAGTTGAGGCCATCAAGGCTGCCCTGCTAAAACATGGACT TGGCAACAGGGTCTCTGTGATGAGCTATAGTGCCAAATTTGCCTCCTGTTTCTACGGTCCTTTCCGGGAT GCAGCTCAGTCAAGCCCAGCTTTTGGAGACCGACGCTGTTATCAGCTGCCTCCTGGTGCCCGTGGCCTGG CCCTCCGAGCAGTGGCCCGAGACATTCAAGAAGGAGCTGACATGCTCATGGTGAAGCCGGGATTGCCCTA CCTGGACATGGTGCGAGAGGTGAAGGACAAGCACCCCGAGCTCCCCCTCGCAGTATACCAGGTGTCTGGA GAGTTTGCCATGTTGTGGCACGGAGCCCAGGCCGGGGCCTTTGATCTCAGGACTGCTGTACTGGAGACCA TGACGGCCTTCCGCAGAGCCGGTGCCGACATCATCATCACCTACTTTGCACCGCAGCTGTTGAAGTGGCT GAAGGAAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204863 representing NM_008525
Red=Cloning site Green=Tags(s) MHHQSVLHSGYFHPLLRSWQTAASTVSASNLIYPIFVTDVPDDVQPIASLPGVARYGVNQLEEMLRPLVE AGLRCVLIFGVPSRVPKDEQGSAADSEDSPTIEAVRLLRKTFPSLLVACDVCLCPYTSHGHCGLLSENGA FLAEESRQRLAEVALAYAKAGCQVVAPSDMMDGRVEAIKAALLKHGLGNRVSVMSYSAKFASCFYGPFRD AAQSSPAFGDRRCYQLPPGARGLALRAVARDIQEGADMLMVKPGLPYLDMVREVKDKHPELPLAVYQVSG EFAMLWHGAQAGAFDLRTAVLETMTAFRRAGADIIITYFAPQLLKWLKEE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008525 |
ORF Size | 990 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008525.4 |
RefSeq Size | 1392 bp |
RefSeq ORF | 993 bp |
Locus ID | 17025 |
UniProt ID | P10518 |
Gene Summary | Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205467 | Alad (untagged) - Mouse aminolevulinate, delta-, dehydratase (Alad), (10ug) |
CNY 2,400.00 |
|
MR204863 | Alad (Myc-DDK-tagged) - Mouse aminolevulinate, delta-, dehydratase (Alad) |
CNY 2,400.00 |
|
MR204863L3 | Lenti ORF clone of Alad (Myc-DDK-tagged) - Mouse aminolevulinate, delta-, dehydratase (Alad) |
CNY 4,750.00 |
|
MR204863L4 | Lenti ORF clone of Alad (mGFP-tagged) - Mouse aminolevulinate, delta-, dehydratase (Alad) |
CNY 4,750.00 |