Neurod4 (NM_007501) Mouse Tagged ORF Clone

CAT#: MG204851

  • TrueORF®

Neurod4 (tGFP-tagged) - Mouse neurogenic differentiation 4 (Neurod4)

ORF Plasmid: DDK tGFP



  "NM_007501" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI846749; ATH-3; Ato; Atoh3; bHLH; bHLHa4; Math; MATH-; MATH-3; Math3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204851 representing NM_007501
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAAAATGTATATGAAATCCAAGGACATGGTGGAGCTGGTCAACACACAATCCTGGATGGACAAAG
GTCTGAGCTCTCAAAATGAGATGAAGGAGCAAGAGAGAAGACCGGGCTCTTATGGAATGCTCGGAACCTT
AACTGAAGAGCATGACAGTATTGAGGAGGATGAAGAAGAGGAAGAAGATGGAGATAAACCTAAAAGAAGA
GGTCCCAAGAAAAAGAAGATGACTAAAGCTCGCCTTGAAAGATTCAGGGCTCGAAGAGTCAAGGCCAATG
CTAGAGAACGGACCCGGATGCATGGCCTGAATGATGCCTTGGATAATCTTAGGAGAGTCATGCCATGTTA
CTCTAAAACTCAAAAGCTTTCCAAGATAGAGACTCTTCGACTGGCAAGGAACTACATCTGGGCCTTGTCT
GAAGTCCTGGAGACTGGTCAGACACTTGAAGGGAAGGGATTTGTAGAGATGCTATGTAAAGGTCTCTCTC
AACCCACAAGCAACCTGGTTGCTGGATGCCTCCAACTGGGGCCTCAATCTACCCTCCTGGAGAAGCATGA
GGAAAAATCTTCAATTTGTGACTCTACTATCTCTGTCCACAGCTTCAACTATCAGTCTCCAGGGCTCCCC
AGCCCTCCTTATGGCCATATGGAAACACATTCTCTCCATCTCAAGCCTCAACCATTTAAGAGTTTGGGTG
ACTCTTTTGGGAGCCATCCACCTGACTGCAGTACCCCCCCTTATGAGGGTCCACTCACACCACCCCTGAG
CATTAGTGGCAACTTCTCCTTAAAGCAAGACGGCTCCCCTGATTTGGAAAAATCCTACAATTTCATGCCA
CATTATACCTCTGCAAGTCTAAGTTCAGGGCATGTGCATTCAACTCCCTTTCAGACTGGCACTCCCCGCT
ATGATGTTCCTGTAGACCTGAGCTATGATTCCTACTCCCACCATAGCATTGGAACTCAGCTCAATACGAT
CTTCTCTGAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204851 representing NM_007501
Red=Cloning site Green=Tags(s)

MAKMYMKSKDMVELVNTQSWMDKGLSSQNEMKEQERRPGSYGMLGTLTEEHDSIEEDEEEEEDGDKPKRR
GPKKKKMTKARLERFRARRVKANARERTRMHGLNDALDNLRRVMPCYSKTQKLSKIETLRLARNYIWALS
EVLETGQTLEGKGFVEMLCKGLSQPTSNLVAGCLQLGPQSTLLEKHEEKSSICDSTISVHSFNYQSPGLP
SPPYGHMETHSLHLKPQPFKSLGDSFGSHPPDCSTPPYEGPLTPPLSISGNFSLKQDGSPDLEKSYNFMP
HYTSASLSSGHVHSTPFQTGTPRYDVPVDLSYDSYSHHSIGTQLNTIFSD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007501
ORF Size 990 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007501.5
RefSeq Size 3376 bp
RefSeq ORF 993 bp
Locus ID 11923
UniProt ID O09105
Gene Summary This gene belongs to the neurogenic differentiation factor family and encodes a basic helix-loop-helix (bHLH) transcription factor which is expressed in the developing nervous system with high levels of expression in the brain, retina and cranial ganglions. Expression gradually becomes restricted to the neural retina. It is a key gene in the Ngn2-regulated neuronal differentiation pathway, coordinating the onset of cortical gene transcription. This gene also regulates amacrine cell fate determination in the retina. [provided by RefSeq, Jul 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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