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Mkrn1 (BC003329) Mouse Tagged ORF Clone
CAT＃: MG204844
- TrueORF®
Mkrn1 (tGFP-tagged) - Mouse makorin, ring finger protein, 1 (cDNA clone MGC:7132 IMAGE:3158135)
ORF Plasmid: DDK
"BC003329" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204844 representing BC003329
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCTGCGGCTCCCGGAACAACAGCCACAACATCAGGAGCAGGAGCGGCAGCGGCGGCGGTGG CAGCGGCCTCCCTCACCTCTATCCCCACAGTCGCTGCCCCGTCCCCGGGGGCGGGAGGAGGGGGCGGCGG CAGCGACGGCAGCGGCGGCGGCTGGACGAAACAGGTCACCTGCAGATACTTTATGCATGGGGTTTGTAAG GAAGGCGATAACTGTCGATACTCTCATGACCTCTCTGACAGTCCATACGGCGTAGTGTGCAAGTATTTTC AAAGAGGGTACTGTGTTTATGGAGACCGCTGCAGATACGAACACAGCAAGCCACTGAAACAGGAAGAAGT GACTGCTACTGATCTGAGTGCAAAACCATCCCTCGCTGCTTCCTCAAGTCTCTCTTCTGGTGTTGGATCA CTTGCTGAAATGAATTCAGGCGAAGCTGAGTCACGAAACCCAAGCTTTCCAACTGTAGGAGCAGGTTCAG AGGACTGGGTGAATGCCATTGAGTTTGTCCCTGGGCAGCCCTACTGTGGCCGTACTGCCCCTTCCTGCAC TGAAGTACCCCCGCAGGGTTCAGTGACCAAGGAAGAGTCAGAGAAGGAGCCAACTACGGTGGAGACAAAG AAGCAGCTTTGCCCCTATGCTGCAGTGGGAGAGTGTCGCTATGGGGAAAACTGTGTGTATCTCCACGGAG ACTCGTGTGACATGTGTGGCCTGCAGGTCCTACACCCAGTGGATGCTGCCCAGAGGTCACAGCACATAAA ATCTTGCATTGAGGCCCATGAGAAGGACATGGAGCTCTCTTTTGCTGTTCAGCGCAGCAAGGACATGGTG TGTGGGATCTGCATGGAGGTGGTCTATGAGAAAGCCAACCCCAGCGAGCGTCGCTTTGGGATCCTCTCCA ACTGCAACCACACCTACTGTCTCAAGTGTATTCGCAAGTGGAGAAGTGCTAAACAATTTGAGAGCAAGAT CATAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204844 representing BC003329
Red=Cloning site Green=Tags(s) MAEAAAPGTTATTSGAGAAAAAVAAASLTSIPTVAAPSPGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCK EGDNCRYSHDLSDSPYGVVCKYFQRGYCVYGDRCRYEHSKPLKQEEVTATDLSAKPSLAASSSLSSGVGS LAEMNSGEAESRNPSFPTVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEVPPQGSVTKEESEKEPTTVETK KQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPVDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMV CGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC003329 |
| ORF Size | 989 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC003329, AAH03329 |
| RefSeq Size | 1523 bp |
| RefSeq ORF | 989 bp |
| Locus ID | 54484 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218107 | Mkrn1 (untagged) - Mouse makorin, ring finger protein, 1 (cDNA clone MGC:7132 IMAGE:3158135), (10ug) |
CNY 6,940.00 |
|
| MR204844 | Mkrn1 (Myc-DDK-tagged) - Mouse makorin, ring finger protein, 1 (cDNA clone MGC:7132 IMAGE:3158135) |
CNY 3,990.00 |
|
| MR204844L3 | Lenti ORF clone of Mkrn1 (Myc-DDK-tagged) - Mouse makorin, ring finger protein, 1 (cDNA clone MGC:7132 IMAGE:3158135) |
CNY 5,890.00 |
|
| MR204844L4 | Lenti ORF clone of Mkrn1 (mGFP-tagged) - Mouse makorin, ring finger protein, 1 (cDNA clone MGC:7132 IMAGE:3158135) |
CNY 5,890.00 |
