Trib3 (BC012955) Mouse Tagged ORF Clone

CAT#: MG204806

  • TrueORF®

Trib3 (tGFP-tagged) - Mouse tribbles homolog 3 (Drosophila) (cDNA clone MGC:18731 IMAGE:3980838)

ORF Plasmid: DDK tGFP



  "BC012955" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Trb3, Nipk, SINK, SKIP3, TRB-3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204806 representing BC012955
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGAGCTACACCTCTGGCTGCTTCTGCTGATGTTTCCTGCAGGAAGAAACCGTTGGAGTTTGATGACA
ATATTGATGCCAAGTGTCCAGTCCTAAAACGAGTGAGAGATGAGCCTGAGCCCGGACCACTCCCCAGCCT
GCTGCCCCCCAGCCCACCTCCCGCCTCAGACTTGTCACCTGCTGTGGCCCCTGCAACTCGACTGGGGCCT
TATATCCTTTTGGAACGAGAGCAAGGCAGCTGCAGCTATCGAGCCCTGCACTGCCCCACAGGCACAGAGT
ACACCTGCAAGGTGTACCCTGCCAGCGAGGCCCAGGCGGTGCTGGCACCTTATGCCCGGCTGCCTACCCA
CCAGCATGTGGCCCGTCCCACAGAGGTCCTGCTGGGCTCTCGGCTCCTTTACATCTTTTTCACGAAGACC
CATGGGGACTTGCACAGCCTGGTGCGCAGCCGCCGCGGTATCCCAGAGCCCGAGGCTGCCGGGCTCTTCC
GGCAGATGGCTAGTGCCGTGGCACACTGCCACAAGCACGGGCTTGTCTTGCGCGACCTCAAGCTGCGTCG
CTTTGTCTTCAGCAACTGTGAGAGGACGAAGCTGGTGCTGGAGAACCTGGAAGATGCCTGCGTGATGACT
GGATCAGATGACTCTCTGTGGGACAAGCATGCGTGCCCTGCCTACGTGGGACCAGAGATACTCAGCTCCC
GGCCATCCTACTCTGAGCCAGTCCTGCTCTTTGGCAAGATCCGTAGAGGGACCTTTGCCCTGCCTGAGGG
CCTATCAGCCCCAGCCCGCTGTCTGATCCGCTGTCTCCTCCGCAAGGAACCTTCAGAGCGACTTGTGGCC
CTGGGAATCCTCTTGCATCCCTGGTTGAGAGAGGATCACGGCCGAGTCTCTCCTCCACAGTCTGACCGAA
GGGAGATGGACCAGGTGGTCCCAGATGGGCCACAGCTGGAGGAGGCTGAGGAAGGGGAGGTGGGGCTGTA
CGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204806 representing BC012955
Red=Cloning site Green=Tags(s)

MRATPLAASADVSCRKKPLEFDDNIDAKCPVLKRVRDEPEPGPLPSLLPPSPPPASDLSPAVAPATRLGP
YILLEREQGSCSYRALHCPTGTEYTCKVYPASEAQAVLAPYARLPTHQHVARPTEVLLGSRLLYIFFTKT
HGDLHSLVRSRRGIPEPEAAGLFRQMASAVAHCHKHGLVLRDLKLRRFVFSNCERTKLVLENLEDACVMT
GSDDSLWDKHACPAYVGPEILSSRPSYSEPVLLFGKIRRGTFALPEGLSAPARCLIRCLLRKEPSERLVA
LGILLHPWLREDHGRVSPPQSDRREMDQVVPDGPQLEEAEEGEVGLYG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC012955
ORF Size 984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC012955, AAH12955
RefSeq Size 1969 bp
RefSeq ORF 986 bp
Locus ID 228775
Gene Summary Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (By similarity). Can inhibit APOBEC3A editing of nuclear DNA.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...