Fbl (BC003813) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	FIB, FLRN, RNU3IP1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204782 representing BC003813 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCAGGTTTCAGCCCCCGTGGGGGTGGCTTTGGTGGCAGAGGCGGCTTTGGTGACAGAGGCGGTA GAGGAGGTGGAAGAGGAGGCCGAGGAGGCTTTGGCGGTGGACGAGGAGGCTTTGGCGGTGGAGGTCGAGG TCGAGGCGGAGGGGGTGGTGGCTTCAGGGGACGAGGAGGTGGCGGTGGCCGAGGTGGGGGCTTCCAGTCT GGGGGCAACCGGGGTCGAGGTGGTGGCCGGGGAGGCAAGAGAGGAAACCAGTCAGGGAAGAATGTGATGG TGGAGCCGCATCGTCATGAAGGTGTCTTTATCTGTCGCGGAAAGGAGGATGCCCTTGTCACAAAGAATCT GGTCCCTGGAGAGTCTGTGTATGGAGAGAAGAGAGTCTCTATTTCAGAAGGAGATGACAAAATTGAGTAC AGAGCCTGGAACCCCTTCCGCTCCAAGCTGGCAGCAGCTATCCTGGGCGGCGTAGACCAGATCCACATCA AGCCAGGAGCCAAGGTGCTCTACCTTGGGGCAGCCTCAGGCACCACCGTCTCCCACGTCTCTGATATTGT CGGCCCGGATGGTCTGGTCTACGCAGTTGAGTTCTCCCACCGCTCTGGCCGTGACCTCATCAACTTGGCC AAGAAGAGGACTAACATTATTCCTGTAATTGAAGATGCTCGACACCCACACAAATACCGCATGCTTATCG CAATGGTGGATGTCATCTTTGCCGATGTGGCCCAGCCAGACCAAACCCGAATTGTGGCCCTGAACGCCCA CACCTTCCTGCGGAATGGAGGACACTTTGTGATTTCCATTAAGGCCAACTGCATTGACTCCACTGCGTCA GCAGAGGCTGTGTTTGCATCTGAAGTGAAGAAGATGCAGCAGGAGAACATGAAGCCGCAGGAGCAGCTGA CGCTAGAGCCTTATGAGCGAGACCACGCCGTGGTTGTCGGTGTGTACAGGCCACCTCCCAAGGTGAAGAA C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204782 representing BC003813 Red=Cloning site Green=Tags(s) MKPGFSPRGGGFGGRGGFGDRGGRGGGRGGRGGFGGGRGGFGGGGRGRGGGGGGFRGRGGGGGRGGGFQS GGNRGRGGGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEY RAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLA KKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTAS AEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN TRTRPLE - GFP Tag - V
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC003813
ORF Size	981 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003813, AAH03813
RefSeq Size	1200 bp
RefSeq ORF	983 bp
Locus ID	14113
Gene Summary	S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).[UniProtKB/Swiss-Prot Function]

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