Plpp3 (NM_080555) Mouse Tagged ORF Clone
CAT#: MG204441
- TrueORF®
Ppap2b (tGFP-tagged) - Mouse phosphatidic acid phosphatase type 2B (Ppap2b)
ORF Plasmid: DDK
"NM_080555" in other vectors (4)
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CNY 4,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1110003O22Rik; 2610002D05Rik; AV025606; D4Bwg0538e; D4Bwg1535e; Lpp3; Ppab2b; Ppap2b |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204441 representing NM_080555
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAGCTACAAGTACGACAAGGCGATCGTCCCTGAGAGTAAGAACGGCGGCAGCCCGGCGCTCAACA ACAACCCGAGGAAGGGCGGCAGCAAGCGGGTGCTGCTCATCTGCCTGGACCTCTTCTGCCTCTTCATGGC GGCTCTGCCCTTCCTCATCATCGAGACAAGCACCATTAAGCCTTACCGTCGAGGGTTTTACTGCAATGAC GAGAGCATCAAGTATCCCCTGAAAGTCAGTGAGACTATAAACGATGCTGTGCTCTGTGCGGTGGGGATCG TCATCGCCATCCTGGCGATCATTACAGGGGAATTCTACCGGATCTATTACCTCAAGGAGAAGTCCCGCTC CACCACTCAGAACCCGTATGTGGCAGCTCTCTATAAGCAAGTGGGATGCTTCCTTTTCGGCTGTGCCATT AGCCAGTCCTTCACCGACATCGCCAAAGTGTCCATCGGGCGCCTGAGGCCTCACTTCCTGAGCGTCTGTG ACCCTGATTTCAGTCAGATCAATTGCTCCGAGGGCTACATTCAGAACTACAGGTGCAGAGGAGAAGACAG CAAAGTACAGGAGGCCAGGAAGTCCTTCTTCTCGGGCCACGCCTCCTTCTCCATGTTCACTATGCTGTAT CTGGTGCTCTACCTTCAGGCCCGCTTCACCTGGCGCGGGGCCCGACTGCTCCGCCCCCTCCTGCAGTTCA CTTTGCTCATGATGGCCTTCTACACGGGATTGTCACGGGTATCTGACTACAAGCATCATCCTAGCGATGT CCTGGCAGGATTTGCCCAAGGAGCTCTGGTGGCCTGCTGCATAGTGTTCTTCGTGTCCGACCTCTTCAAG ACTAAGACGAGCCTCTCACTGCCCGCCCCTGCGATCAGGAGGGAGATCCTGTCTCCCGTGGACATCATCG ACAGGAACAATCACCATAACATGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204441 representing NM_080555
Red=Cloning site Green=Tags(s) MQSYKYDKAIVPESKNGGSPALNNNPRKGGSKRVLLICLDLFCLFMAALPFLIIETSTIKPYRRGFYCND ESIKYPLKVSETINDAVLCAVGIVIAILAIITGEFYRIYYLKEKSRSTTQNPYVAALYKQVGCFLFGCAI SQSFTDIAKVSIGRLRPHFLSVCDPDFSQINCSEGYIQNYRCRGEDSKVQEARKSFFSGHASFSMFTMLY LVLYLQARFTWRGARLLRPLLQFTLLMMAFYTGLSRVSDYKHHPSDVLAGFAQGALVACCIVFFVSDLFK TKTSLSLPAPAIRREILSPVDIIDRNNHHNMV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_080555 |
ORF Size | 936 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_080555.2, NP_542122.1 |
RefSeq Size | 3162 bp |
RefSeq ORF | 939 bp |
Locus ID | 67916 |
UniProt ID | Q99JY8 |
Gene Summary | Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P) (By similarity). Essential to the formation of the chorioallantoic placenta and extraembryonic vasculature. Also mediates gastrulation and axis formation, probably by regulating the Wnt signaling pathway.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200411 | Ppap2b (untagged) - Mouse phosphatidic acid phosphatase type 2B (Ppap2b), (10ug) |
CNY 2,400.00 |
|
MR204441 | Ppap2b (Myc-DDK-tagged) - Mouse phosphatidic acid phosphatase type 2B (Ppap2b) |
CNY 2,400.00 |
|
MR204441L3 | Lenti ORF clone of Ppap2b (Myc-DDK-tagged) - Mouse phosphatidic acid phosphatase type 2B (Ppap2b) |
CNY 4,750.00 |
|
MR204441L4 | Lenti ORF clone of Ppap2b (mGFP-tagged) - Mouse phosphatidic acid phosphatase type 2B (Ppap2b) |
CNY 4,750.00 |