Cdk5r1 (BC050768) Mouse Tagged ORF Clone

CAT#: MG204342

  • TrueORF®

Cdk5r1 (tGFP-tagged) - Mouse cyclin-dependent kinase 5, regulatory subunit (p35) 1 (cDNA clone MGC:58293 IMAGE:6533538)



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Mouse Protein / Lysate Request

CNY 5,200.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Cdk5r1 Antibody - C-terminal region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms p35, Cdk5r, p25
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204342 representing BC050768
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCACGGTGCTGTCCCTATCCCCCAGCTATCGGAAGGCCACACTGTTTGAGGATGGCGCGGCCACCG
TGGGCCACTACACGGCCGTGCAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGGCACTCCATCAT
CTCGGTGCTGCCTTGGAAGAGGATCGTGGCGGTGTCAGCGAAGAAGAAGAACTCCAAGAAGGCGCAGCCC
AACAGCAGCTACCAGAGCAACATCGCGCATCTCAACAATGAGAACCTGAAGAAGTCGCTGTCCTGTGCCA
ACCTGTCCACATTCGCCCAGCCCCCGCCGGCTCAGCCGCCCGCACCCCCAGCCAGCCAGCTTTCTGGCTC
CCAGACTGGGGTCTCCTCTTCTGTCAAGAAGGCCCCGCACCCTGCCATCACCTCTGCAGGGACACCCAAA
CGGGTCATCGTCCAGGCGTCCACTAGTGAGCTGCTGCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCT
ACCGCCTGAAGCACTTGTCCCCAACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCCCTGCTTCT
GCAGGGCTGGCAGGACCAGGGTTTCATCACACCGGCCAATGTGGTCTTCCTCTACATGCTCTGCAGGGAT
GTCATCTCCTCTGAGGTGGGCTCCGACCATGAGCTCCAGGCTGTCCTGCTGACCTGTCTGTACCTCTCCT
ATTCCTACATGGGCAATGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTAGAGAGCTGTAAGGAAGCCTT
TTGGGACCGTTGCCTCTCAGTTATCAACCTCATGAGCTCCAAGATGCTGCAGATCAATGCCGACCCACAC
TATTTCACACAAGTGTTCTCTGACTTGAAGAATGAGAGCGGTCAGGAGGACAAGAAGCGACTCCTCCTGG
GGCTGGATCGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204342 representing BC050768
Red=Cloning site Green=Tags(s)

MGTVLSLSPSYRKATLFEDGAATVGHYTAVQNSKNAKDKNLKRHSIISVLPWKRIVAVSAKKKNSKKAQP
NSSYQSNIAHLNNENLKKSLSCANLSTFAQPPPAQPPAPPASQLSGSQTGVSSSVKKAPHPAITSAGTPK
RVIVQASTSELLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPANVVFLYMLCRD
VISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESCKEAFWDRCLSVINLMSSKMLQINADPH
YFTQVFSDLKNESGQEDKKRLLLGLDR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC050768
ORF Size 921 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC050768, AAH50768
RefSeq Size 4166 bp
RefSeq ORF 923 bp
Locus ID 12569
Gene Summary p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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