Ppp4c (NM_019674) Mouse Tagged ORF Clone
CAT#: MG204327
- TrueORF®
Ppp4c (tGFP-tagged) - Mouse protein phosphatase 4, catalytic subunit (Ppp4c)
ORF Plasmid: DDK
"NM_019674" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1110002D08Rik; AU016079; Ppx |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204327 representing NM_019674
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGATCAGCGACCTGGACCGGCAGATCGAGCAGCTGCGGCGCTGCGAGCTGATCAAAGAGAGTG AAGTCAAGGCCCTGTGCGCCAAGGCCAGAGAAATCTTGGTAGAAGAGAGCAACGTGCAGAGGGTGGACTC GCCAGTCACAGTATGCGGTGACATCCATGGACAATTCTATGACCTCAAGGAGCTGTTCAGAGTAGGTGGC GATGTCCCTGAGACCAACTACCTCTTCATGGGAGACTTTGTGGACCGTGGTTTCTACAGTGTTGAAACCT TCCTCCTCCTGCTGGCTCTTAAGGTTCGCTATCCTGACAGAATCACTTTGATCCGGGGCAATCATGAGAG TCGCCAGATTACCCAGGTCTATGGGTTCTACGATGAGTGCTTACGGAAATATGGTTCAGTGACTGTATGG CGCTACTGTACTGAGATCTTTGACTACCTCAGCCTGTCTGCCATCATTGATGGCAAGATCTTCTGTGTGC ATGGAGGTCTTTCCCCTTCCATCCAGACCTTGGACCAGATCCGGACAATTGACCGAAAGCAAGAGGTACC CCATGATGGACCCATGTGCGACCTCCTGTGGTCTGACCCTGAAGACACAACAGGCTGGGGAGTGAGCCCC CGCGGGGCAGGTTACCTGTTTGGCAGTGACGTGGTCGCCCAGTTCAATGCAGCCAACGACATTGATATGA TCTGCCGTGCCCACCAATTAGTGATGGAAGGCTACAAGTGGCACTTCAATGAGACCGTGCTTACTGTGTG GTCAGCGCCTAATTACTGCTACCGCTGTGGCAATGTGGCAGCCATCTTAGAACTGGATGAGCACCTCCAG AAAGATTTCATCATCTTCGAGGCTGCACCCCAAGAGACACGTGGCATCCCCTCCAAAAAGCCAGTGGCCG ACTATTTCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204327 representing NM_019674
Red=Cloning site Green=Tags(s) MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVTVCGDIHGQFYDLKELFRVGG DVPETNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSVTVW RYCTEIFDYLSLSAIIDGKIFCVHGGLSPSIQTLDQIRTIDRKQEVPHDGPMCDLLWSDPEDTTGWGVSP RGAGYLFGSDVVAQFNAANDIDMICRAHQLVMEGYKWHFNETVLTVWSAPNYCYRCGNVAAILELDEHLQ KDFIIFEAAPQETRGIPSKKPVADYFL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_019674 |
ORF Size | 921 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019674.2 |
RefSeq Size | 1332 bp |
RefSeq ORF | 924 bp |
Locus ID | 56420 |
UniProt ID | P97470 |
Gene Summary | Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair (By similarity). Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207523 | Ppp4c (untagged) - Mouse protein phosphatase 4, catalytic subunit (Ppp4c), (10ug) |
CNY 3,990.00 |
|
MR204327 | Ppp4c (Myc-DDK-tagged) - Mouse protein phosphatase 4, catalytic subunit (Ppp4c) |
CNY 2,400.00 |
|
MR204327L3 | Lenti ORF clone of Ppp4c (Myc-DDK-tagged) - Mouse protein phosphatase 4, catalytic subunit (Ppp4c) |
CNY 4,750.00 |
|
MR204327L4 | Lenti ORF clone of Ppp4c (mGFP-tagged) - Mouse protein phosphatase 4, catalytic subunit (Ppp4c) |
CNY 4,750.00 |