Nmnat2 (NM_175460) Mouse Tagged ORF Clone
CAT#: MG204325
- TrueORF®
Nmnat2 (tGFP-tagged) - Mouse nicotinamide nucleotide adenylyltransferase 2 (Nmnat2)
ORF Plasmid: DDK
"NM_175460" in other vectors (4)
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CNY 4,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI843915; D030041I09Rik; PNAT1; PNAT2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204325 representing NM_175460
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGAGACCACAAAGACCCACGTTATCCTGCTGGCCTGCGGCAGCTTCAATCCCATCACTAAAGGGC ACATTCAGATGTTCGAGAGAGCCAGGGATTATCTGCACAAGACTGGAAGATTTATTGTGATTGGCGGGAT TGTCTCTCCGGTCCATGACTCCTACGGAAAACAGGGCCTTGTGTCAAGTCGGCACCGTCTCATCATGTGT CAGCTGGCTGTCCAGAATTCCGACTGGATCAGGGTGGACCCATGGGAGTGCTATCAGGACACCTGGCAGA CAACCTGCAGTGTGTTGGAGCACCATCGAGACCTGATGAAGAGGGTGACCGGCTGCATCCTCTCCAACGT CAACACACCTTCCATGACACCTGTGATCGGACAGCCACAGCATGAGAACACCCAGCCCATTTACCAGAAC AGCAATGTGCCCACCAAGCCCACTGCAGCCAAGATCTTGGGAAAGGTGGGAGAAAGCCTCAGCCGGATCT GCTGCGTCCGCCCACCAGTGGAGCGCTTCACTTTTGTAGATGAGAACGCCAACCTGGGCACAGTGATGCG GTATGAGGAGATCGAGCTGCGCATCTTGCTGCTGTGTGGTAGTGACCTGCTGGAGTCTTTCTGCATCCCA GGACTCTGGAATGAGGCAGATATGGAAGTGATTGTTGGGGACTTTGGGATCGTCGTGGTACCCAGGGATG CAGCGGACACAGACCGGATCATGAATCACTCCTCCATACTCCGCAAGTACAAAAACAACATCATGGTTGT GAAGGATGATATCAACCATCCCATGTCTGTAGTCAGCTCCACCAAGAGCAGGCTGGCCCTGCAGCATGGG GACGGCCATGTTGTGGATTACCTGTCCCAGCCAGTCATCGATTACATCCTCAAGAGTCAGCTGTACATCA ACGCCTCGGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204325 representing NM_175460
Red=Cloning site Green=Tags(s) MTETTKTHVILLACGSFNPITKGHIQMFERARDYLHKTGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMC QLAVQNSDWIRVDPWECYQDTWQTTCSVLEHHRDLMKRVTGCILSNVNTPSMTPVIGQPQHENTQPIYQN SNVPTKPTAAKILGKVGESLSRICCVRPPVERFTFVDENANLGTVMRYEEIELRILLLCGSDLLESFCIP GLWNEADMEVIVGDFGIVVVPRDAADTDRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSTKSRLALQHG DGHVVDYLSQPVIDYILKSQLYINASG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_175460 |
ORF Size | 921 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175460.3, NP_780669.1 |
RefSeq Size | 4548 bp |
RefSeq ORF | 924 bp |
Locus ID | 226518 |
UniProt ID | Q8BNJ3 |
Gene Summary | Nicotinamide/nicotinate-nucleotide adenylyltransferase that acts as an axon maintenance factor (PubMed:20126265, PubMed:23082226). Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (By similarity). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency (By similarity). Cannot use triazofurin monophosphate (TrMP) as substrate (By similarity). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (By similarity). For the pyrophosphorolytic activity prefers NAD(+), NADH and NaAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively (By similarity). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (By similarity). Axon survival factor required for the maintenance of healthy axons: acts by delaying Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons (PubMed:20126265, PubMed:23082226).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212784 | Nmnat2 (untagged) - Mouse nicotinamide nucleotide adenylyltransferase 2 (Nmnat2), (10ug) |
CNY 2,400.00 |
|
MR204325 | Nmnat2 (Myc-DDK-tagged) - Mouse nicotinamide nucleotide adenylyltransferase 2 (Nmnat2) |
CNY 2,400.00 |
|
MR204325L3 | Lenti ORF clone of Nmnat2 (Myc-DDK-tagged) - Mouse nicotinamide nucleotide adenylyltransferase 2 (Nmnat2) |
CNY 4,750.00 |
|
MR204325L4 | Lenti ORF clone of Nmnat2 (mGFP-tagged) - Mouse nicotinamide nucleotide adenylyltransferase 2 (Nmnat2) |
CNY 4,800.00 |