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Hus1 (BC061249) Mouse Tagged ORF Clone
CAT＃: MG204186
- TrueORF®
Hus1 (tGFP-tagged) - Mouse Hus1 homolog (S. pombe) (cDNA clone MGC:74405 IMAGE:30249148)
ORF Plasmid: DDK
"BC061249" in other vectors (4)
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CNY 3,330.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | mHus1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204186 representing BC061249
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTTCGCGCCAAGATCGTGGACCTGGCTTGTCTGAATCATTTCACACGAGTCAGTAACATGATAG CCAAGCTTGCCAAAACCTGCACCCTCCGCATCAGCCCGGAGAAGCTGAACTTCATCCTTTGCGACAAGCT GGCCAGTGGAGGCGTGAGCATGTGGTGTGAGCTGGAGCAGGAGAACTTTTTTAGTGAATTTCAAATGGAA GGAGTCTCTGAAGAAAACAACGAGATTTATTTAGAATTAACGTCGGAAAACTTATCTCGAGCCTTGAAAA CTGCCCAGAACTCCAGAGCCTTGAAAATCAAGCTGACTAACAAACACTTTCCCTGTCTTACCGTGTCTGT AGAGCTGCAGGTGTCTTCATCGAGCAGCAGCAGAATCGTGGTGCATGATATCCCCATAAAGGTTCTTCCG AGAAGACTGTGGAAGGACTTACAAGAACCCTCCATCCCAGACTGTGATGTCAGTATTTGCTTACCAGCCT TGAAGATGATGAAGAGTGTTGTGGAAAAAATGAGAAACATCAGCAATCAGCTTGTGATTGAAGCAAACCT AAAGGGAGAATTAAACCTAAAGATAGAAACTGAGTTAGTGTGTGTGACTACTCATTTTAAGGATCTTGAA AACCCTCTATTACCCTCTGACAGTGTCTCTCAAAACAGACACCCAGAAGACATGGCCAAGGTGCACATTG ACATAAAGAAACTCCTCCAGTTTCTTGCCGGACAGCAAGTGACTCCCACCAAGGCAGTGTGCAGTGAGTT TGCTTCTCCATTATCCACCTCCTTTGTCCTACATATGCAAGTATGCCCAGTTCTGGCAATCGAGCTGCCT GAGACAGTCAGGCTTGAAAGGTCCCTTGAGCCAGAGGTATCTAGAGATGCTGAGGAAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204186 representing BC061249
Red=Cloning site Green=Tags(s) MKFRAKIVDLACLNHFTRVSNMIAKLAKTCTLRISPEKLNFILCDKLASGGVSMWCELEQENFFSEFQME GVSEENNEIYLELTSENLSRALKTAQNSRALKIKLTNKHFPCLTVSVELQVSSSSSSRIVVHDIPIKVLP RRLWKDLQEPSIPDCDVSICLPALKMMKSVVEKMRNISNQLVIEANLKGELNLKIETELVCVTTHFKDLE NPLLPSDSVSQNRHPEDMAKVHIDIKKLLQFLAGQQVTPTKAVCSEFASPLSTSFVLHMQVCPVLAIELP ETVRLERSLEPEVSRDAEEE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC061249 |
| ORF Size | 900 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC061249.1 |
| RefSeq Size | 2127 bp |
| RefSeq ORF | 902 bp |
| Locus ID | 15574 |
| Gene Summary | This gene encodes a component of a cell cycle checkpoint complex that causes cell cycle arrest in response to bulky DNA lesions and DNA replication blockage. Together with the proteins Rad9 and Rad1, the encoded protein forms a heterotrimeric complex known as the 9-1-1 complex. Mice lacking the encoded protein develop spontaneous chromosomal abnormalities resulting in embryonic lethality. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218182 | Hus1 (untagged) - Mouse Hus1 homolog (S. pombe) (cDNA clone MGC:74405 IMAGE:30249148), (10ug) |
CNY 6,940.00 |
|
| MR204186 | Hus1 (Myc-DDK-tagged) - Mouse Hus1 homolog (S. pombe) (cDNA clone MGC:74405 IMAGE:30249148) |
CNY 3,040.00 |
|
| MR204186L3 | Lenti ORF clone of Hus1 (Myc-DDK-tagged) - Mouse Hus1 homolog (S. pombe) (cDNA clone MGC:74405 IMAGE:30249148) |
CNY 4,940.00 |
|
| MR204186L4 | Lenti ORF clone of Hus1 (mGFP-tagged) - Mouse Hus1 homolog (S. pombe) (cDNA clone MGC:74405 IMAGE:30249148) |
CNY 4,940.00 |
