Ccdc98 (BC029845) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	3830405G04Rik; 5630400M01Rik; AI506069; AL024423; AV118690; Ccdc98
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204136 representing BC029845 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATTTAGAGAGCAGTTACTCCACAGAAACTTACAGACACATCTTTCAAGCCCTGAGCTGGTTTTCC TGTTGTTAACACCGAGTATAACAACAGAAAGCTGCTCCACTCACTGCCTGGAACACGCCTTATATAAACC TCAAAGGGGGCTTTTTCATAGGGTACCATTGGTGGTTACCAATCTGGGAATGTCTGATCAACTGGGTTAT AAAACAGAACCTGCTTCCTGTACATCCACTGTCTTTAGTAGAGCTGTGAGGACACACAGTTCTCAGTTCT TTAATGAAGATGGATCATTAAAGGAAGTCCATAAGATAAATGAAATGTATGCTGCTGTACAAGAAGAGTT AAAGAGTATATGCCAAAAAGTTGAACAAAGTGAACGAGAAGTAGAGAAACTATTGATGGATGTAAACCAA TTAAAAGAAGTTAGAAGAACCCAGCAAGCACGGGCTACAGGAGCAGGAGAGAAGAATGTCCAGAGAAACC CACAGGAGAACATCCTTCTTTGTCAAGCTTTGCGAACATTTTTTCCAGAGTCTGAAGTTCTTCATTCTTG CGTTATTTCTTTGAAAAATAGACACATTTCTCCCAGTGGGTGTAACGTTAACCACCATGTTGATGTAGTG GATCAGCTGACCTTAATGGTAGAGTATGTGTACAGCCCTGAAGCTAGTCCAGTGCCCACAGCCCAGCTAC GTAAACGGAAGGCTCTAGACACACAGGACCAGGGGTCTGTGAAGAGACCACGGCTGCTGGAGACAGAAAG CAGACCTTCTGTGGCAGCGTCCCGCAGCAGGCACCAAGACAAGGCTTCCTCAAGCAGCCTAGATATAGAC ATAGAGATGGGGAGCCCAGAAGATGATGCTGACTATCCTCGCTCTCCCACATTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204136 representing BC029845 Red=Cloning site Green=Tags(s) MTFREQLLHRNLQTHLSSPELVFLLLTPSITTESCSTHCLEHALYKPQRGLFHRVPLVVTNLGMSDQLGY KTEPASCTSTVFSRAVRTHSSQFFNEDGSLKEVHKINEMYAAVQEELKSICQKVEQSEREVEKLLMDVNQ LKEVRRTQQARATGAGEKNVQRNPQENILLCQALRTFFPESEVLHSCVISLKNRHISPSGCNVNHHVDVV DQLTLMVEYVYSPEASPVPTAQLRKRKALDTQDQGSVKRPRLLETESRPSVAASRSRHQDKASSSSLDID IEMGSPEDDADYPRSPTF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC029845
ORF Size	896 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029845, AAH29845
RefSeq Size	1680 bp
RefSeq ORF	896 bp
Locus ID	70681
Gene Summary	Involved in DNA damage response and double-strand break (DSB) repair. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and mediates the recruitment of BRCA1. The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at DSBs. This complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX.[UniProtKB/Swiss-Prot Function]

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