Fbxo2 (NM_176848) Mouse Tagged ORF Clone
CAT#: MG204114
- TrueORF®
Fbxo2 (tGFP-tagged) - Mouse F-box protein 2 (Fbxo2)
ORF Plasmid: DDK
"NM_176848" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | FBG1; Fbs1; Fbs2; FBX2; NFB42; Prpl4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204114 representing NM_176848
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGAGATGGTGATCCAGAGAGTGTGAGCCACCCCGAAGAAGCGAGCCCAGAGGAGCAGCCAGAGG AGGCGGGCGCCGAGGCGAGTGCGGAGGAGGAGCAGCTCCGGGAGGCGGAGGAGGAGGAAGAGGCGGAGGC CGTGGAGTACCTGGCCGAGCTGCCGGAGCCACTGCTGCTGCGCGTGCTGGCCGAGCTGCCAGCTACGGAG CTGGTGCAGGCCTGCCGCCTGGTGTGCCTGCGCTGGAAGGAGCTGGTGGACGGCGCCCCACTGTGGCTGC TCAAGTGCCAGCAGGAGGGGCTGGTGCCCGAGGGCAGCGCTGATGAGGAGCGGGACCACTGGCAACAGTT CTACTTTCTGAGCAAGAGGAGGCGCAACCTGCTGCGCAACCCTTGTGGGGAAGAGGACTTGGAGGGCTGG AGCGACGTGGAGCACGGTGGGGACGGCTGGAGGGTGGAGGAACTGCCCGGAGACAATGGGGTGGAATTTA CCCAAGATGACAGCGTTAAGAAATACTTCGCCTCCTCCTTCGAGTGGTGTCGCAAAGCGCAGGTCATTGA TCTGCAGGCAGAGGGCTACTGGGAGGAGCTACTGGACACCACCCAGCCCGCCATCGTGGTGAAGGACTGG TACTCGGGTCGCACTGATGCGGGCAGCCTGTATGAGCTCACTGTGAGGCTGCTGTCGGAGAACGAAGATG TGCTGGCGGAGTTCGCTACAGGACAGGTGGCTGTGCCAGAGGACGGCAGTTGGATGGAGATCTCCCACAC CTTCATCGACTACGGGCCAGGTGTCCGCTTTGTCCGCTTCGAGCACGGAGGGCAGGACTCGGTCTACTGG AAGGGCTGGTTCGGGGCCCGGGTGACCAACAGTAGCGTGTGGGTGGAACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204114 representing NM_176848
Red=Cloning site Green=Tags(s) MDGDGDPESVSHPEEASPEEQPEEAGAEASAEEEQLREAEEEEEAEAVEYLAELPEPLLLRVLAELPATE LVQACRLVCLRWKELVDGAPLWLLKCQQEGLVPEGSADEERDHWQQFYFLSKRRRNLLRNPCGEEDLEGW SDVEHGGDGWRVEELPGDNGVEFTQDDSVKKYFASSFEWCRKAQVIDLQAEGYWEELLDTTQPAIVVKDW YSGRTDAGSLYELTVRLLSENEDVLAEFATGQVAVPEDGSWMEISHTFIDYGPGVRFVRFEHGGQDSVYW KGWFGARVTNSSVWVEP TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_176848 |
ORF Size | 891 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_176848.1, NP_789818.1 |
RefSeq Size | 1260 bp |
RefSeq ORF | 894 bp |
Locus ID | 230904 |
UniProt ID | Q80UW2 |
Gene Summary | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Prevents formation of cytosolic aggregates of unfolded glycoproteins that have been retrotranslocated into the cytosol. Able to recognize and bind denatured glycoproteins, preferentially those of the high-mannose type.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203502 | Fbxo2 (untagged) - Mouse F-box protein 2 (Fbxo2), (10ug) |
CNY 2,400.00 |
|
MR204114 | Fbxo2 (Myc-DDK-tagged) - Mouse F-box protein 2 (Fbxo2) |
CNY 2,400.00 |
|
MR204114L3 | Lenti ORF clone of Fbxo2 (Myc-DDK-tagged) - Mouse F-box protein 2 (Fbxo2) |
CNY 4,750.00 |
|
MR204114L4 | Lenti ORF clone of Fbxo2 (mGFP-tagged) - Mouse F-box protein 2 (Fbxo2) |
CNY 4,750.00 |