United States
Germany
Japan
United Kingdom
China
Nhej1 (NM_029342) Mouse Tagged ORF Clone
CAT＃: MG204061
- TrueORF®
Nhej1 (tGFP-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1)
ORF Plasmid: DDK
"NM_029342" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700029B21Rik; cernunnos; XLF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204061 representing NM_029342
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGCTAGAGCAAGACCTGTTGCTGCAGCCATGGGCATGGTTACAACTTGCGGAGAACTCACTCT TAGCCAAGGTGTCTATCACCAAGCACGGTTATGCCTTGCTGATTTCGGATCTTCAACAGGTGTGGCATGA ACAGGTGGACACTTCGGTGGTCAGCCAGCGAGCCAAGGAGCTGAACAAGCGCCTCACTGCGCCTCCTGCA GCTTTGCTCTGTCACCTGGATGAAGCACTTCGCCCACTGTTTAAAGATTCTGCTCACCCTAGCAAAGCTA CTTTCTCCTGTGACCGAGGAGAGGAGGGACTGATCCTGCGGGTGCAGAGTGAGCTCTCGGGTCTTCCCTT CAGTTGGCATTTCCACTGTATTCCAGCTAGTTCTTCACTGGTCTCTCAGCATTTGATTCATCCTCTGATG GGTGTGAGCCTGGCACTGCAGAGTCATGTGAGGGAGCTAGCAGCATTGCTTCGGATGAAGGACCTTGAGA TCCAGGCCTACCAGGAGAGTGGGGCTGTGCTGAGCCGAAGTCGATTGAAGACAGAGCCATTTGAAGAAAA TTCTTTCTTGGAACAGTTTATGGCAGAGAAATTGCCAGAGGCGTGTGCTGTTGGTGATGGAAAGCCATTT GCCATGAGTCTGCAGAGTCTGTATGTGGCAGTTACAAAACAGCAGATCCAAGCAAGGCAGGCACATAAAG ACTCTGGAGAGACTCAGGCATCAAGCAGCACCTCCCCTCGAGGAACTGATAACCAGCCAGAAGAGCCGGT CTCCCTCCCTTCCACCCTCTCAGAACCTGAATATGAGCCTGTGGCTGCTTCAGGCCCTATGCATAGAGCT CAGCTGGTGAAGTCCAAGAGGAAGAAGCCCAGGGGACTCTTCAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204061 representing NM_029342
Red=Cloning site Green=Tags(s) MEELEQDLLLQPWAWLQLAENSLLAKVSITKHGYALLISDLQQVWHEQVDTSVVSQRAKELNKRLTAPPA ALLCHLDEALRPLFKDSAHPSKATFSCDRGEEGLILRVQSELSGLPFSWHFHCIPASSSLVSQHLIHPLM GVSLALQSHVRELAALLRMKDLEIQAYQESGAVLSRSRLKTEPFEENSFLEQFMAEKLPEACAVGDGKPF AMSLQSLYVAVTKQQIQARQAHKDSGETQASSSTSPRGTDNQPEEPVSLPSTLSEPEYEPVAASGPMHRA QLVKSKRKKPRGLFS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029342 |
| ORF Size | 885 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029342.2 |
| RefSeq Size | 1401 bp |
| RefSeq ORF | 888 bp |
| Locus ID | 75570 |
| UniProt ID | Q3KNJ2 |
| Gene Summary | DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary (PubMed:17360556). Binds DNA in a length-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200480 | Nhej1 (untagged) - Mouse nonhomologous end-joining factor 1 (Nhej1), (10ug) |
CNY 2,000.00 |
|
| MR204061 | Nhej1 (Myc-DDK-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) |
CNY 2,081.00 |
|
| MR204061L3 | Lenti ORF clone of Nhej1 (Myc-DDK-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) |
CNY 4,750.00 |
|
| MR204061L4 | Lenti ORF clone of Nhej1 (mGFP-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) |
CNY 4,750.00 |
