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Nhej1 (NM_029342) Mouse Untagged Clone
CAT＃: MC200480
Nhej1 (untagged) - Mouse nonhomologous end-joining factor 1 (Nhej1), (10ug)
CNY 2,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1700029B21Rik; cernunnos; XLF |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC006063 sequence for NM_029342
CTCTCAGTTTGCGCACCAGGACAGGCCCGCCTTGAGGAGGGTGGGAGTTCCCGCAGCCGTTGACGTTGAC ATGGAGGTGTGAGCCGCCCTCTTGAAGGCTGACTTTCCTGAGGACACTGTGCTGAATTAGGACTTTTCTG CAGATGGAAGAGCTAGAGCAAGACCTGTTGCTGCAGCCATGGGCATGGTTACAACTTGCGGAGAACTCAC TCTTAGCCAAGGTGTCTATCACCAAGCACGGTTATGCCTTGCTGATTTCGGATCTTCAACAGGTGTGGCA TGAACAGGTGGACACTTCGGTGGTCAGCCAGCGAGCCAAGGAGCTGAACAAGCGCCTCACTGCGCCTCCT GCAGCTTTGCTCTGTCACCTGGATGAAGCACTTCGCCCACTGTTTAAAGATTCTGCTCACCCTAGCAAAG CTACTTTCTCCTGTGACCGAGGAGAGGAGGGACTGATCCTGCGGGTGCAGAGTGAGCTCTCGGGTCTTCC CTTCAGTTGGCATTTCCACTGTATTCCAGCTAGTTCTTCACTGGTCTCTCAGCATTTGATTCATCCTCTG ATGGGTGTGAGCCTGGCACTGCAGAGTCATGTGAGGGAGCTAGCAGCATTGCTTCGGATGAAGGACCTTG AGATCCAGGCCTACCAGGAGAGTGGGGCTGTGCTGAGCCGAAGTCGATTGAAGACAGAGCCATTTGAAGA AAATTCTTTCTTGGAACAGTTTATGGCAGAGAAATTGCCAGAGGCGTGTGCTGTTGGTGATGGAAAGCCA TTTGCCATGAGTCTGCAGAGTCTGTATGTGGCAGTTACAAAACAGCAGATCCAAGCAAGGCAGGCACATA AAGACTCTGGAGAGACTCAGGCATCAAGCAGCACCTCCCCTCGAGGAACTGATAACCAGCCAGAAGAGCC GGTCTCCCTCCCTTCCACCCTCTCAGAACCTGAATATGAGCCTGTGGCTGCTTCAGGCCCTATGCATAGA GCTCAGCTGGTGAAGTCCAAGAGGAAGAAGCCCAGGGGACTCTTCAGTTAACTCTCCATGTCTCAGCCAC CACGAAGAGAGCAAAACCACTTCCTAGCATCACCTGGAAGGAGCTTGCATGGCAGAGCATCCCTCAGAGG CCTGAGTTTGTGTTGACAGGCCACCTGGATGGGGACTTTACCAGAGGAAGCCTGACTCCAACTCACTGGC ATTCCGGATATGAGTGACACCAGCTCCTTGCTTGGGTAGTGTTCTTTCTTTAGAACTAGCACCCTATGGC AAACTTAGGTACATGAAGCAAGTTTACAAAGAGGCGCATGCCCAGAACTCCTGGCTGCCTTTCACAGGGG TCTCTCTCTCTCAACACAGAATAAAGGATGCTCACACTCCTCGACTGTTGAGGTTACCTGTGATCTCTGG ATCCCAGAATCCCCCTAGAACAGAACTGGGTGTCCCCTAGTCCTGTTGGGTCACATCTGAGCTATCAGCT CCATCCTACAGACTCTTCGTCAGCTTCTCTCATGAGGCTGAGGTCAGCCACAGTTAGAAGCCTTGATCTT GAGAACTCTCTCCATGTTGGTACAAGACAGATTACATTTCTGTCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_029342 |
| Insert Size | 888 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC006063, AAH06063 |
| RefSeq Size | 1634 bp |
| RefSeq ORF | 888 bp |
| Locus ID | 75570 |
| UniProt ID | Q3KNJ2 |
| Gene Summary | DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary (PubMed:17360556). Binds DNA in a length-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204061 | Nhej1 (tGFP-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) |
CNY 2,850.00 |
|
| MR204061 | Nhej1 (Myc-DDK-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) |
CNY 2,400.00 |
|
| MR204061L3 | Lenti ORF clone of Nhej1 (Myc-DDK-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) |
CNY 4,750.00 |
|
| MR204061L4 | Lenti ORF clone of Nhej1 (mGFP-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1) |
CNY 4,750.00 |
