Josd3 (BC110660) Mouse Tagged ORF Clone
CAT#: MG203916
- TrueORF®
Josd3 (tGFP-tagged) - Mouse Josephin domain containing 3 (cDNA clone MGC:117821 IMAGE:3158156)
ORF Plasmid: DDK
"BC110660" in other vectors (4)
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CNY 2,850.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 5930426I11Rik, 2810003M17Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203916 representing BC110660
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAATCAGAAGTGAATTCTGTCTGTGTGGCATCGGATAGAGCTGGAGATACTGGAGACCAAAGTG ATGACTCCTCTGATGGCAGCTTATTTAAAACTCAGTGTGCTCCTTCACCTATACAAAAGCAAAGACACCC TACTGTGAAACGTGTTACTTTACCTGCAAGTGTTGAAACAGATTCATCAAGTGACTCTTCTATAGAACCA AGGCCTCTGACTTTAAAGGCTATTTTTGAAAGATTTAAAAAAAAGAAACGGAAAAAGAGGAAAAAGAGAA AATATGAGCCAAAATTAAGACCAAGAGGAAGACCAAGAGGAAAACCATCGGGAACGAGGATCACTAGAAG GTCGCAAATAGATGCAAAACAAATTAAAGACAAAGGAGCTGTGTTTCCATTCTTAGAATCTGAGAGTGGA AGAAAACCTTTACCTTGGAAGAAAATTTTAACATATGAGCAAGCTGTTGCAAGAGGATTTTTTCACCACA TTGAAAAACTCAAGTATGAACACCACCTTAAGGAATGCTTGAAGCAAATGCATGCTGGGGAAGATTTAGA AAAGGAAGACCTTGACAGTCGGAGGCACAAATACATGGATGATGATGGTTCACTTTCTCCTATTGAAGAG CCATTAACTGAAGATGAAGCAACAAATCCTCAGGCTGAATGTGATATCAAGTTGGTTGAAGACAGTTGCT TCATAATAAGTTCAGAATTCTCAAGGAAGAGAAACTTGGAACAAGAGAAGATTAAGAAAGAATCTACATT TTCTAAAAAAGCCAAAGATGCCACACACAGAGAGAAAGGGCACAGAAGAACCTTGAAAGGAAATGAACAT GTGACTATTGAAGAAGGCTTGTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203916 representing BC110660
Red=Cloning site Green=Tags(s) MAQSEVNSVCVASDRAGDTGDQSDDSSDGSLFKTQCAPSPIQKQRHPTVKRVTLPASVETDSSSDSSIEP RPLTLKAIFERFKKKKRKKRKKRKYEPKLRPRGRPRGKPSGTRITRRSQIDAKQIKDKGAVFPFLESESG RKPLPWKKILTYEQAVARGFFHHIEKLKYEHHLKECLKQMHAGEDLEKEDLDSRRHKYMDDDGSLSPIEE PLTEDEATNPQAECDIKLVEDSCFIISSEFSRKRNLEQEKIKKESTFSKKAKDATHREKGHRRTLKGNEH VTIEEGLS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC110660 |
ORF Size | 866 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC110660, AAI10661 |
RefSeq Size | 2254 bp |
RefSeq ORF | 866 bp |
Locus ID | 75316 |
Gene Summary | Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217912 | Josd3 (untagged) - Mouse Josephin domain containing 3 (cDNA clone MGC:117821 IMAGE:3158156), (10ug) |
CNY 6,940.00 |
|
MR203916 | Josd3 (Myc-DDK-tagged) - Mouse Josephin domain containing 3 (cDNA clone MGC:117821 IMAGE:3158156) |
CNY 2,400.00 |
|
MR203916L3 | Lenti ORF clone of Josd3 (Myc-DDK-tagged) - Mouse Josephin domain containing 3 (cDNA clone MGC:117821 IMAGE:3158156) |
CNY 4,750.00 |
|
MR203916L4 | Lenti ORF clone of Josd3 (mGFP-tagged) - Mouse Josephin domain containing 3 (cDNA clone MGC:117821 IMAGE:3158156) |
CNY 4,750.00 |