Hes1 (BC051428) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	bHLHb39; Hry
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203818 representing BC051428 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTGATATAATGGAGAAAAATTCCTCCTCCCCGGTGGCTGCTACCCCAGCCAGTGTCAACACGA CACCGGACAAACCAAAGACGGCCTCTGAGCACAGAAAGTCATCAAAGCCTATCATGGAGAAGAGGCGAAG GGCAAGAATAAATGAAAGTCTAAGCCAACTGAAAACACTGATTTTGGATGCACTTAAGAAAGATAGCTCC CGGCATTCCAAGCTAGAGAAGGCAGACATTCTGGAAATGACTGTGAAGCACCTCCGGAACCTGCAGCGGG CGCAGATGACCGCCGCGCTCAGCACAGACCCGAGCGTGTTGGGGAAATACCGCGCCGGCTTCAGCGAGTG CATGAACGAGGTGACCCGCTTCCTGTCCACGTGTGAGGGCGTTAACACCGAGGTGCGCACTCGGCTGCTG GGCCACCTGGCCAACTGCATGACCCAGATCAACGCCATGACCTACCCCGGGCAGGCGCACCCCGCCTTGC AGGCGCCGCCGCCGCCGCCCCCGTCAGGACCTGCCGGTCCCCAGCACGCGCCATTCGCGCCGCCGCCGCC GCCGCTTGTGCCCATCCCCGGGGGCGCGGCGCCCCCTCCCGGCAGCGCACCCTGCAAGTTGGGCAGCCAG GCTGGAGAGGCTGCCAAGGTTTTTGGCGGCTTCCAAGTGGTGCCGGCTCCTGACGGCCAATTTGCCTTTC TCATCCCCAACGGGGCCTTCGCTCACAGCGGCCCGGTCATCCCGGTCTACACCAGCAACAGTGGGACCTC GGTGGGTCCTAACGCAGTGTCACCTTCCAGTGGCTCCTCGCTCACTTCGGACTCCATGTGGAGACCGTGG CGGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203818 representing BC051428 Red=Cloning site Green=Tags(s) MPADIMEKNSSSPVAATPASVNTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSS RHSKLEKADILEMTVKHLRNLQRAQMTAALSTDPSVLGKYRAGFSECMNEVTRFLSTCEGVNTEVRTRLL GHLANCMTQINAMTYPGQAHPALQAPPPPPPSGPAGPQHAPFAPPPPPLVPIPGGAAPPPGSAPCKLGSQ AGEAAKVFGGFQVVPAPDGQFAFLIPNGAFAHSGPVIPVYTSNSGTSVGPNAVSPSSGSSLTSDSMWRPW RN TRTRPLE - GFP Tag - V
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC051428
ORF Size	846 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC051428, AAH51428
RefSeq Size	1527 bp
RefSeq ORF	848 bp
Locus ID	15205
Gene Summary	Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1 (By similarity). Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage (By similarity).[UniProtKB/Swiss-Prot Function]

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