Hes1 (BC051428) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	bHLHb39; Hry
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC051428 AGCTGGTGCTGATAACAGCGGAATCCCCTGTCTACCTCTCTCCTTGGTCCTGGAATAGTGCTACCGATCA CTAAGTAGCCCTAAGACATAATAAACCTTCAACTGCTCAGTAGTTTTTCTTATGAAAGTCAAGTAAAAGG ACGTAAGCAAAAAAAAATTATTTTTTTTTTTGCGTGAAGGATTCCAAAAATAAAATTCTCTGGGGACTGA GAAAAAAAAAAAAAAAAAAACGAAAATGCCAGCTGATATAATGGAGAAAAATTCCTCCTCCCCGGTGGCT GCTACCCCAGCCAGTGTCAACACGACACCGGACAAACCAAAGACGGCCTCTGAGCACAGAAAGTCATCAA AGCCTATCATGGAGAAGAGGCGAAGGGCAAGAATAAATGAAAGTCTAAGCCAACTGAAAACACTGATTTT GGATGCACTTAAGAAAGATAGCTCCCGGCATTCCAAGCTAGAGAAGGCAGACATTCTGGAAATGACTGTG AAGCACCTCCGGAACCTGCAGCGGGCGCAGATGACCGCCGCGCTCAGCACAGACCCGAGCGTGTTGGGGA AATACCGCGCCGGCTTCAGCGAGTGCATGAACGAGGTGACCCGCTTCCTGTCCACGTGTGAGGGCGTTAA CACCGAGGTGCGCACTCGGCTGCTGGGCCACCTGGCCAACTGCATGACCCAGATCAACGCCATGACCTAC CCCGGGCAGGCGCACCCCGCCTTGCAGGCGCCGCCGCCGCCGCCCCCGTCAGGACCTGCCGGTCCCCAGC ACGCGCCATTCGCGCCGCCGCCGCCGCCGCTTGTGCCCATCCCCGGGGGCGCGGCGCCCCCTCCCGGCAG CGCACCCTGCAAGTTGGGCAGCCAGGCTGGAGAGGCTGCCAAGGTTTTTGGCGGCTTCCAAGTGGTGCCG GCTCCTGACGGCCAATTTGCCTTTCTCATCCCCAACGGGGCCTTCGCTCACAGCGGCCCGGTCATCCCGG TCTACACCAGCAACAGTGGGACCTCGGTGGGTCCTAACGCAGTGTCACCTTCCAGTGGCTCCTCGCTCAC TTCGGACTCCATGTGGAGACCGTGGCGGAACTGAGAGCCTCAGGCCACTGCTACCCGTAAAGTCCCTAGC CCACCTCTCTCTTCTGACGGACACTAAAAACGAACTTGGATTTTAGGAGAGACTTTTATAATTTGGTGGT TATTTTGTTGCTTTTTTTAATTCTAAAAAGTTACTTTTTGTAGAGAGCTGTATTAAGTGACTGACCATGC ACTGCATTTGTATATATTTTATATGTTCATATTGGATTGCGCCTTTGTATTATAAAAGTTGAGATGACAT TTCGTTTTTTACACGAGATTTCTTTTTTTATGTGATGCCAAAGATGTTTGAAAATGCTCTTAAAATATCT TCCTTTGGGGAAGTTTATTTGAGAAAATATAATAAAAGAGTGAAGGCTTTTAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC051428
Insert Size	849 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC051428, AAH51428
RefSeq Size	1527 bp
RefSeq ORF	849 bp
Locus ID	15205
Gene Summary	Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1 (By similarity). Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage (By similarity).[UniProtKB/Swiss-Prot Function]

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