Dhrs4 (BC054361) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mouNRDR, RRD
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203776 representing BC054361 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAAAGCGGGACGGCTGTTAGGTGGCTGGACTCAAGCGTGGATGTCGGTGAGGATGGCCAGTTCCG GGTTGACTCGTCGAAACCCTCTCTCGAATAAGGTGGCCTTGGTCACAGCCTCCACCGACGGGATCGGCTT TGCCATCGCCCGTCGTCTGGCTGAAGATGGGGCCCACGTGGTCGTCAGCAGCCGCAAACAGCAGAATGTG GACCGTGCAGTGGCCACACTACAGGGAGAGGGCCTGAGTGTGACTGGCATCGTGTGCCACGTGGGGAAGG CAGAGGATCGAGAAAAGCTGATAACCACGGCTCTGAAGCGTCACCGGGGGATTGATATCCTGGTCTCCAA TGCTGCTGTCAACCCTTTCTTTGGAAATCTAATGGATGTCACAGAGGAGGTGTGGGACAAGGTTTTGAGC ATTAATGTGACAGCTACAGCCATGATGATTAAAGCGGTGGTGCCAGAGATGGAAAAGCGAGGAGGCGGCT CAGTGGTGATTGTGGGTTCTGTAGCAGGCTTCACTCGGTTCCCTTCTCTGGGTCCTTACAATGTTAGCAA AACAGCTTTGCTGGGTCTTACTAAGAACTTTGCAGCGGAGTTGGCCCCGAAGAACATTCGAGTGAACTGC TTAGCACCTGGACTCATCAAGACTCGATTCAGCAGTGTGTTGTGGGAGGAGAAAGCAAGAGAGGACTTCA TAAAAGAAGCCATGCAAATCAGAAGGCTAGGCAAGCCAGAGGATTGTGCTGGCATAGTTTCCTTCTTATG CTCTGAAGACGCCAGTTACATCAATGGAGAGACCGTAGTAGTGGGGGGAGGAACCCCTTCTCGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203776 representing BC054361 Red=Cloning site Green=Tags(s) MQKAGRLLGGWTQAWMSVRMASSGLTRRNPLSNKVALVTASTDGIGFAIARRLAEDGAHVVVSSRKQQNV DRAVATLQGEGLSVTGIVCHVGKAEDREKLITTALKRHRGIDILVSNAAVNPFFGNLMDVTEEVWDKVLS INVTATAMMIKAVVPEMEKRGGGSVVIVGSVAGFTRFPSLGPYNVSKTALLGLTKNFAAELAPKNIRVNC LAPGLIKTRFSSVLWEEKAREDFIKEAMQIRRLGKPEDCAGIVSFLCSEDASYINGETVVVGGGTPSRL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC054361
ORF Size	839 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC054361, AAH54361
RefSeq Size	1375 bp
RefSeq ORF	839 bp
Locus ID	28200
Gene Summary	Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.