Aurkc (BC064780) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AIK3, AIE1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203721 representing BC064780 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCAGCACCTCAACCAGGAAGCATTTCACCATCAATGACTTTGAAATCGGGCGTCCTCTGGGCA GGGGGAAATTTGGGCGTGTGTACTTGGCTCGGCTCAAGGAAAATCATTTCATCGTGGCCCTGAAGGTCCT CTTCAAGTCTGAGATAGAGAAGGAGGGATTGGAGCACCAACTTCGCAGGGAAGTGGAGATCCAGGCACAC CTACAACACCGGAACATCCTTCGCCTGTACAACTACTTCTATGATGACACTCGGATATACTTAATTCTGG AGTATGCTCCAGGAGGAGAGCTCTATAAGGAGCTTCAGAGACATCAGAAGTTGGACCAGCAGCGTACAGC CACGATAATACAGGAGTTGTCAGATGCCCTGACCTACTGCCATGAGAAGAAGGTGATTCACAGGGACATC AAGCCAGAGAATCTCCTGCTGGGCCTCAATGGTGAGGTGAAGATCTCAGACTTTGGGTGGTCTGTGCATA CCCCCTCTCTCAGGAGAAAGACAATGTGTGGGACTCTGGACTACTTGCCCCCGGAAATGATAGCGCAGAA ACCGTACAATGAGATGGTTGATCTGTGGTGCATTGGGGTGCTCTGCTATGAGCTGCTGGTGGGGAAGCCA CCCTTTGAGAGCAGCACCTCCAGTGAGACATACAGACGCATCCGCCAGGTGGATTTTAAGTTTCCTTCAT CAGTACCTGCAGGAGCCCAGGACTTGATCTCCAAGCTTCTTAGGTACCATCCTTCAGAGCGGCTGAGCCT GGCCCAGGTCCTGAAGCACCCCTGGGTCAGGGAACACTCTCGAAGGGTGCTTCCTTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203721 representing BC064780 Red=Cloning site Green=Tags(s) MEPSTSTRKHFTINDFEIGRPLGRGKFGRVYLARLKENHFIVALKVLFKSEIEKEGLEHQLRREVEIQAH LQHRNILRLYNYFYDDTRIYLILEYAPGGELYKELQRHQKLDQQRTATIIQELSDALTYCHEKKVIHRDI KPENLLLGLNGEVKISDFGWSVHTPSLRRKTMCGTLDYLPPEMIAQKPYNEMVDLWCIGVLCYELLVGKP PFESSTSSETYRRIRQVDFKFPSSVPAGAQDLISKLLRYHPSERLSLAQVLKHPWVREHSRRVLPC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC064780
ORF Size	830 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC064780, AAH64780
RefSeq Size	1358 bp
RefSeq ORF	830 bp
Locus ID	20871
Gene Summary	Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Plays also a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. AURKC phosphorylates the CPC complex subunits BIRC5/survivin and INCENP leading to increased AURKC activity. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.[UniProtKB/Swiss-Prot Function]

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