Hvcn1 (NM_028752) Mouse Tagged ORF Clone
CAT#: MG203573
- TrueORF®
Hvcn1 (tGFP-tagged) - Mouse hydrogen voltage-gated channel 1 (Hvcn1), transcript variant 2
"NM_028752" in other vectors (2)
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CNY 4,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 0610039P13Rik; AI450555; BTS; HV1; mVSOP; Vsop. |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203573 representing NM_028752
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTCCCATGACCCAAAGGCCGTCACTCGCAGAACCAAGGTGGCTCCCACCAAGAGGATGAGCAGGT TCTTGAAACACTTTACGGTGGTTGGGGACGACTACCACACGTGGAATGTCAACTACAAGAAGTGGGAGAA TGAGGAGGAGGAGGAGGAGCCAGCGCCCACATCAGCAGAGGGTGAGGGCAATGCTGAGGGCCCAGATGCC GAGGCTGGCTCTGCCTCCACGCCCAGGCAGTCCCTGGACTTCAGGAGCCGACTGAGGAAACTCTTCAGTT CCCACAGGTTTCAGGTCATCATCATCTGCCTGGTGGTCCTGGACGCCCTCCTCGTGCTTGCTGAACTCCT CCTGGATTTGAAGATCATCGAGCCGGACGAGCAAGACTATGCGGTCACGGCGTTCCACTACATGAGCTTT GCCATCCTGGTCTTCTTCATGTTGGAGATTTTTTTCAAGATCTTCGTCTTCCGCTTAGAGTTCTTCCACC ACAAGTTTGAGATCCTGGATGCCTTCGTGGTGGTGGTGTCTTTCGTCCTTGACCTTGTCCTCTTGTTTAA AAGCCACCACTTCGAAGCTCTAGGGCTGCTGATCTTGCTTCGGCTCTGGAGGGTGGCCCGGATCATCAAT GGCATCATCATCTCCGTGAAGACACGCTCAGAACGGCAGATCTTAAGGCTAAAGCAGATAAATATCCAAC TGGCCACCAAGATCCAGCATCTGGAATTCAGCTGCTCCGAGAAGGAACAAGAAATTGAGCGGCTCAACAA GCTGTTGAAACAGAATGGACTTCTCGGGGACGTGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203573 representing NM_028752
Red=Cloning site Green=Tags(s) MTSHDPKAVTRRTKVAPTKRMSRFLKHFTVVGDDYHTWNVNYKKWENEEEEEEPAPTSAEGEGNAEGPDA EAGSASTPRQSLDFRSRLRKLFSSHRFQVIIICLVVLDALLVLAELLLDLKIIEPDEQDYAVTAFHYMSF AILVFFMLEIFFKIFVFRLEFFHHKFEILDAFVVVVSFVLDLVLLFKSHHFEALGLLILLRLWRVARIIN GIIISVKTRSERQILRLKQINIQLATKIQHLEFSCSEKEQEIERLNKLLKQNGLLGDVN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_028752 |
ORF Size | 807 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028752.3, NP_083028.1 |
RefSeq Size | 2641 bp |
RefSeq ORF | 810 bp |
Locus ID | 74096 |
UniProt ID | Q3U2S8 |
Gene Summary | Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.[UniProtKB/Swiss-Prot Function] |
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