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Penk (NM_001002927) Mouse Tagged ORF Clone
CAT＃: MG203555
- TrueORF®
Penk (tGFP-tagged) - Mouse preproenkephalin 1 (Penk1)
ORF Plasmid: DDK
"NM_001002927" in other vectors (4)
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CNY 5,200.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI326464; ENK; Penk1; PPA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203555 representing NM_001002927
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGTTCCTGAGGCTTTGCACCTGGCTGCTGGCGCTTGGGTCCTGCCTCCTGGCTACAGTGCAGG CGGAATGCAGCCAGGACTGCGCTAAATGCAGCTACCGCCTGGTTCGCCCAGGCGACATCAATTTCCTGGC GTGCACACTGGAATGTGAAGGACAGCTGCCTTCTTTCAAAATCTGGGAGACCTGCAAGGATCTCCTGCAG GTGTCCAGGCCCGAGTTCCCTTGGGATAACATCGACATGTACAAAGACAGCAGCAAACAGGATGAGAGCC ACTTGCTAGCCAAGAAGTACGGAGGCTTCATGAAACGGTACGGAGGCTTCATGAAGAAGATGGACGAGCT ATATCCCATGGAGCCAGAAGAAGAAGCGAACGGAGGAGAGATCCTTGCCAAGAGGTATGGCGGCTTCATG AAGAAGGATGCAGATGAGGGAGACACCTTGGCCAACTCCTCCGATCTGCTGAAAGAGCTACTGGGAACGG GAGACAACCGTGCGAAAGACAGCCACCAACAAGAGAGCACCAACAATGACGAAGACATGAGCAAGAGGTA TGGGGGCTTCATGAGAAGCCTCAAAAGAAGCCCCCAACTGGAAGATGAAGCAAAAGAGCTGCAGAAGCGC TACGGGGGCTTCATGAGAAGGGTGGGACGCCCCGAGTGGTGGATGGACTACCAGAAGAGGTATGGGGGCT TCCTGAAGCGCTTTGCTGAGTCTCTGCCCTCCGATGAAGAAGGCGAAAATTACTCGAAAGAAGTTCCTGA GATAGAGAAAAGATACGGGGGCTTTATGCGGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203555 representing NM_001002927
Red=Cloning site Green=Tags(s) MARFLRLCTWLLALGSCLLATVQAECSQDCAKCSYRLVRPGDINFLACTLECEGQLPSFKIWETCKDLLQ VSRPEFPWDNIDMYKDSSKQDESHLLAKKYGGFMKRYGGFMKKMDELYPMEPEEEANGGEILAKRYGGFM KKDADEGDTLANSSDLLKELLGTGDNRAKDSHQQESTNNDEDMSKRYGGFMRSLKRSPQLEDEAKELQKR YGGFMRRVGRPEWWMDYQKRYGGFLKRFAESLPSDEEGENYSKEVPEIEKRYGGFMRF TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001002927 |
| ORF Size | 804 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001002927.3 |
| RefSeq Size | 1443 bp |
| RefSeq ORF | 807 bp |
| Locus ID | 18619 |
| UniProt ID | P22005 |
| Gene Summary | Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Characterization of Endorphin Gene Expression Following Electroporation in Mice Tibialis Anterior Muscle: Implications for Gene Doping Detection
,Nohr, MK;Gazerani, P;Gehl, J;Lichota, J;,
Int J Sports Exerc Med
[PENK]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209162 | Penk (untagged) - Mouse preproenkephalin (Penk), (10ug) |
CNY 3,990.00 |
|
| MR203555 | Penk (Myc-DDK-tagged) - Mouse preproenkephalin (Penk) |
CNY 3,600.00 |
|
| MR203555L3 | Lenti ORF clone of Penk (Myc-DDK-tagged) - Mouse preproenkephalin (Penk) |
CNY 5,890.00 |
|
| MR203555L4 | Lenti ORF clone of Penk (mGFP-tagged) - Mouse preproenkephalin (Penk) |
CNY 5,890.00 |
