Rbm7 (NM_144948) Mouse Tagged ORF Clone

CAT#: MG203518

  • TrueORF®

Rbm7 (tGFP-tagged) - Mouse RNA binding motif protein 7 (Rbm7)

ORF Plasmid: DDK tGFP



  "NM_144948" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1200007M24Rik; 1500011D06Rik; AU041934; AW554393
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG203518 representing NM_144948
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCGGCGGCCGCAGAGGCCGACCGCACTCTGTTCGTGGGTAACCTGGAGACGAAGGTGACAGAGG
AGCTCCTCTTCGAGCTGTTCCACCAGGCTGGGCCGGTAATAAAAGTGAAAATCCCGAAAGATAAAGATGG
CAAACTGAAGCAGTTTGCATTCGTGAACTTCAAACATGAAGTGTCTGTTCCCTATGCCATGAATCTGCTC
AACGGAATCAAACTTTTCGGGAGGCCTATCAAAATTCAGTTTAGATCAGGAAGCAGTCACGCCTCTCAGG
ATGCCAGTGTGTCATATCCCCAGCATCATGTTGGAAATTTAAGCCCAACCTCCACATCTCCTAACAGCTA
TGAAAGGACAGTGGGTAACGTGTCTCCGACAGCTCAGATGGTCCAGAGGTCCTTTTCTTCTCCAGAAGAT
TATCAGCGGCAAGCAGTGATGAACAGTGTTTTCAGACAGATGTCATATGCTGGGAAATTTGGTTCTCCAC
ATGCGGATCAGTTGGGATTTTCACCATCAGCTCAACCACATGGCCATACCTTTAACCAGTCTTCCAGCTC
CCAGTGGCGCCAAGATGCACTGTCATCACAGCGTAAAAGACAGAATTCTCACCCCTACCTAGCAGATAGA
CACTATAGCCGTGAGCAGCGCTACTCTGACCATGGGTCTGACTATCATTACAGAGGCAGCCGAGAGGATT
TCTACTATGACGACAGGGATCATGATGGCTGGAGCCATGACTATGATAACAGAAGGGACAGTAGTAGAGG
TGGGAAGTGGCCCTCATCCAGACAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG203518 representing NM_144948
Red=Cloning site Green=Tags(s)

MGAAAAEADRTLFVGNLETKVTEELLFELFHQAGPVIKVKIPKDKDGKLKQFAFVNFKHEVSVPYAMNLL
NGIKLFGRPIKIQFRSGSSHASQDASVSYPQHHVGNLSPTSTSPNSYERTVGNVSPTAQMVQRSFSSPED
YQRQAVMNSVFRQMSYAGKFGSPHADQLGFSPSAQPHGHTFNQSSSSQWRQDALSSQRKRQNSHPYLADR
HYSREQRYSDHGSDYHYRGSREDFYYDDRDHDGWSHDYDNRRDSSRGGKWPSSRH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_144948
ORF Size 795 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_144948.2, NP_659197.2
RefSeq Size 1774 bp
RefSeq ORF 798 bp
Locus ID 67010
UniProt ID Q9CQT2
Gene Summary Subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters. Possible involved in germ cell RNA processing and meiosis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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