Spin1 (BC016517) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Spin
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG203454 representing BC016517 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACCCCATTCGGGAAGACACCTGGCCAACGGTCCAGAGCTGATGCAGGCCATGCTGGAGTATCTG CAAACATGATGAAGAAGAGGACATCTCACAAAAAACATCGGACCAGTGTGGGACCAAGCAAGCCTGTGTC CCAACCCCGGCGGAACATCGTAGGCTGCAGGATCCAGCATGGATGGAGAGAGGGCAATGGCCCTGTTACC CAGTGGAAGGGGACTGTCCTGGACCAGGTGCCTGTGAATCCTTCCCTGTATCTTATAAAGTACGATGGAT TTGACTGTGTTTATGGACTAGAACTTAATAAGGATGAAAGAGTTTCTGCACTGGAAGTCCTCCCTGATAG AGTTGCAACATCTCGGATCAGCGATGCACACTTAGCGGACACAATGATCGGCAAAGCAGTGGAGCACATG TTTGAGACAGAGGACGGCTCTAAAGATGAGTGGAGGGGGATGGTCTTGGCACGGGCGCCTGTCATGAACA CATGGTTTTACATCACCTATGAGAAAGACCCTGTCTTGTACATGTACCAGCTCCTCGATGACTACAAAGA AGGCGACCTCCGCATCATGCCTGATTCCAATGATTCGCCTCCAGCAGAAAGGGAGCCAGGAGAAGTTGTG GACAGCCTGGTAGGCAAGCAAGTGGAATATGCCAAAGAAGACGGCTCGAAAAGGACTGGCATGGTCATCC ACCAAGTAGAGGCCAAGCCCTCTGTCTATTTCATCAAGTTTGATGACGATTTCCATATTTACGTCTACGA TTTGGTGAAAACATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG203454 representing BC016517 Red=Cloning site Green=Tags(s) MKTPFGKTPGQRSRADAGHAGVSANMMKKRTSHKKHRTSVGPSKPVSQPRRNIVGCRIQHGWREGNGPVT QWKGTVLDQVPVNPSLYLIKYDGFDCVYGLELNKDERVSALEVLPDRVATSRISDAHLADTMIGKAVEHM FETEDGSKDEWRGMVLARAPVMNTWFYITYEKDPVLYMYQLLDDYKEGDLRIMPDSNDSPPAEREPGEVV DSLVGKQVEYAKEDGSKRTGMVIHQVEAKPSVYFIKFDDDFHIYVYDLVKTS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC016517
ORF Size	788 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016517, AAH16517
RefSeq Size	1105 bp
RefSeq ORF	788 bp
Locus ID	20729
Gene Summary	Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (By similarity). Overexpression induces metaphase arrest and chromosomal instability (PubMed:18543248). Localizes to active rDNA loci and promotes the expression of rRNA genes. May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption (PubMed:23894536).[UniProtKB/Swiss-Prot Function]

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