Wdr73 (BC028870) Mouse Tagged ORF Clone

CAT#: MG203438

  • TrueORF®

Wdr73 (tGFP-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)

ORF Plasmid: DDK tGFP



  "BC028870" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,950.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1200011I23Rik; 2410008B13Rik; AI848574; C85352
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG203438 representing BC028870
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCAAGTCAGAGAGGACAACGATGTCATTGAAGCAGTCAGTACCATCGATGTGCAGGACAAAGAGG
AGAGTCTCTGGCCTCGGGTGTCTGTCTTCTGCTCTAAGGCACCAGGAATCCTCCACGGGGCGCGGCTCAG
CGGCCTGAGGACTGTGGATGTAGAATCCCAGAAGATCACATATAGCTCAGGTACAGCTGACAGTGAGTCC
TTGAGCTGCCTTCAGGTCCTAGACGCAAATACCTTTGCTTTCTGTGGCAACTCAGGTCGGCTGGGGCTTG
TGGACACCCGCCAGAAGTGGGCAGCGTTGGAGACTGTCAGCCCCGGCTCTGGCTGTAGTGGAGAGAGATG
GTGTGCAGAAGTCAGGAACAAGGGCCAAGGCCCTGGGCCCTGCATTGCCAGCCTTGGCTCAGATGGGCAG
CTCTGTCTCCTTGACTCCCGGAATCTCTGTCATCCTGTGAGCTCAGCCCAGTGTCCGGTGTTCAAACCCA
GCCCTGACCCAGAGCTGCTGCGGGTGACTTGGGCTCCGGGCCTGGACAACTGCTTGGCTATTTCAGGCTT
TGATGGGACAGTACAAATCTATGATGTCACATCATGGGATGGAAAGAAAACCCAAGCGGAGCCTCTCTTC
ACTCACAAAGGCCACATCTTCCTGGATGGAAATGACGTAGACTCTGCTCCTCTGGTCACCACCCATACCT
GGCACCCTCGCAAACCCAGAACTTTGTTATCAGCAGCAAGTGATTCTTCTCTGCATGTGTGGGACTGGGT
GGACCTCCAGGCCTCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG203438 representing BC028870
Red=Cloning site Green=Tags(s)

MWQVREDNDVIEAVSTIDVQDKEESLWPRVSVFCSKAPGILHGARLSGLRTVDVESQKITYSSGTADSES
LSCLQVLDANTFAFCGNSGRLGLVDTRQKWAALETVSPGSGCSGERWCAEVRNKGQGPGPCIASLGSDGQ
LCLLDSRNLCHPVSSAQCPVFKPSPDPELLRVTWAPGLDNCLAISGFDGTVQIYDVTSWDGKKTQAEPLF
THKGHIFLDGNDVDSAPLVTTHTWHPRKPRTLLSAASDSSLHVWDWVDLQAS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC028870
ORF Size 789 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC028870.1
RefSeq Size 1544 bp
RefSeq ORF 788 bp
Locus ID 71968
Gene Summary May play a role in the regulation of microtubule organization and dynamics.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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