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Pou2af1 (NM_011136) Mouse Tagged ORF Clone
CAT＃: MG203298
- TrueORF®
Pou2af1 (tGFP-tagged) - Mouse POU domain, class 2, associating factor 1 (Pou2af1)
ORF Plasmid: DDK
"NM_011136" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Bob-1; BOB.1; Bob1; OBF-1; OBF.1; OCA-B; OCAB |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203298 representing NM_011136
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTGGCAAAAATCCGCAGCTCCAGAGCAAGCTCCTGCCCCACCAAGGCCACACCAGGGTGTTCGAG TCAAGGAGCCAGTGAAGGAGCTACTGAGAAGAAAGCGTGGCCATACCAGCGTTGGGGCAGCTGGGCCACC GACCGCGGTGGTACTGCCCCACCAGCCCCTGGCCACCTACAGCACTGTGGGTCCTTCCTGCCTTGACATG GAGGTTTCTGCTTCCACAGTGACAGAGGAGGGAACATTATGTGCTGGCTGGCTCTCCCAACCTGCCCCGG CCACTCTTCAGCCATTGGCTCCATGGACACCCTACACGGAGTATGTGTCCCATGAAGCTGTCAGCTGCCC CTACTCCACTGACATGTACGTGCAGCCTGTGTGCCCCAGCTACACAGTGGTGGGACCCTCCTCGGTGTTG ACCTATGCTTCTCCACCACTCATCACTAATGTCACGCCAAGAAGCACTGCTACACCCGCGGTGGGGCCCC AGCTGGAGGGTCCCGAGCACCAGGCGCCCCTCACTTATTTCCCGTGGCCTCAGCCCCTTTCCACACTGCC CACCTCCAGCCTGCAGTATCAACCTCCTGCCCCAACCCTGTCTGGGCCCCAGTTTGTCCAGCTCCCCATC TCTATCCCAGAGCCAGTCCTTCAGGACATGGATGACCCCAGAAGGGCCATCAGCTCCCTGACCATTGACA AGCTGCTTCTGGAGGAAGAGGAAAGCAACACGTACGAGCTCAACCACACCCTCTCCGTGGAGGGCTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203298 representing NM_011136
Red=Cloning site Green=Tags(s) MLWQKSAAPEQAPAPPRPHQGVRVKEPVKELLRRKRGHTSVGAAGPPTAVVLPHQPLATYSTVGPSCLDM EVSASTVTEEGTLCAGWLSQPAPATLQPLAPWTPYTEYVSHEAVSCPYSTDMYVQPVCPSYTVVGPSSVL TYASPPLITNVTPRSTATPAVGPQLEGPEHQAPLTYFPWPQPLSTLPTSSLQYQPPAPTLSGPQFVQLPI SIPEPVLQDMDDPRRAISSLTIDKLLLEEEESNTYELNHTLSVEGF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011136 |
| ORF Size | 768 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011136.2, NP_035266.1 |
| RefSeq Size | 2566 bp |
| RefSeq ORF | 771 bp |
| Locus ID | 18985 |
| UniProt ID | Q64693 |
| Gene Summary | Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201929 | Pou2af1 (untagged) - Mouse POU domain, class 2, associating factor 1 (Pou2af1), (10ug) |
CNY 2,400.00 |
|
| MR203298 | Pou2af1 (Myc-DDK-tagged) - Mouse POU domain, class 2, associating factor 1 (Pou2af1) |
CNY 2,400.00 |
|
| MR203298L3 | Lenti ORF clone of Pou2af1 (Myc-DDK-tagged) - Mouse POU domain, class 2, associating factor 1 (Pou2af1) |
CNY 4,750.00 |
|
| MR203298L4 | Lenti ORF clone of Pou2af1 (mGFP-tagged) - Mouse POU domain, class 2, associating factor 1 (Pou2af1) |
CNY 4,800.00 |
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