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Etfb (BC049237) Mouse Tagged ORF Clone
CAT＃: MG203280
- TrueORF®
Etfb (tGFP-tagged) - Mouse electron transferring flavoprotein, beta polypeptide (cDNA clone MGC:54845 IMAGE:4241552)
ORF Plasmid: DDK
"BC049237" in other vectors (3)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 0610009I16Rik; 2810441H06Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203280 representing BC049237
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCTGCGCGCGCTCGTGGCTGTCAAGAGGGTCATCGACTTCGCTGTGAAGATTCGGGTAAAGC CGGACAAGTCTGGAGTGGTCACTGATGGTGTGAAGCACTCCATGAACCCCTTCTGTGAGATTGCAGTGGA AGAGGCTGTGCGGCTAAAGGAGAAGAAACTGGTGAAGGAGATCATTGCCGTCAGCTGTGGCCCCTCACAG TGCCAGGAGACCATCCGAACTGCTCTGGCCATGGGTGCAGACAGAGGCATCCACGTGGAGATACCAGGGG CACAGGCAGAAAGCTTAGGCCCCCTGCAGGTGGCCCGGGTCCTGGCCAAGCTGGCTGAAAAGGAGAAAGT GGACCTTTTGTTCCTGGGCAAGCAGGCTATTGATGATGACTGTAACCAGACAGGTCAGATGACAGCTGGA CTTCTGGACTGGCCGCAGGGTACATTCGCCTCTCAGGTGACACTGGAGGGGGACAAGGTAAAAGTGGAAC GGGAAATTGACGGGGGTCTGGAGACCCTTCGCCTGAAGCTGCCCGCTGTGGTGACTGCTGACCTAAGGCT CAATGAGCCTCGCTATGCCACCCTGCCCAACATCATGAAAGCCAAGAAGAAGAAGATTGAAGTGGTCAAG GCTGGAGACCTGGGCGTGGACCTGACCTCCAAGGTCTCTGTGATCAGTGTGGAAGAGCCCCCTCAGCGCT CAGCAGGAGTCAAGGTGGAGACCACAGAAGACCTGGTGGCCAAGCTGAAGGAGGTGGGGCGGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203280 representing BC049237
Red=Cloning site Green=Tags(s) MAELRALVAVKRVIDFAVKIRVKPDKSGVVTDGVKHSMNPFCEIAVEEAVRLKEKKLVKEIIAVSCGPSQ CQETIRTALAMGADRGIHVEIPGAQAESLGPLQVARVLAKLAEKEKVDLLFLGKQAIDDDCNQTGQMTAG LLDWPQGTFASQVTLEGDKVKVEREIDGGLETLRLKLPAVVTADLRLNEPRYATLPNIMKAKKKKIEVVK AGDLGVDLTSKVSVISVEEPPQRSAGVKVETTEDLVAKLKEVGRI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC049237 |
| ORF Size | 767 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC049237, AAH49237 |
| RefSeq Size | 835 bp |
| RefSeq ORF | 767 bp |
| Locus ID | 110826 |
| Gene Summary | Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (By similarity). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed:25023281). ETFB binds an AMP molecule that probably has a purely structural role (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR203280 | Etfb (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, beta polypeptide (cDNA clone MGC:54845 IMAGE:4241552) |
CNY 2,400.00 |
|
| MR203280L3 | Lenti ORF clone of Etfb (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, beta polypeptide (cDNA clone MGC:54845 IMAGE:4241552) |
CNY 4,750.00 |
|
| MR203280L4 | Lenti ORF clone of Etfb (mGFP-tagged) - Mouse electron transferring flavoprotein, beta polypeptide (cDNA clone MGC:54845 IMAGE:4241552) |
CNY 4,750.00 |
