Ccng1 (BC005534) Mouse Tagged ORF Clone

CAT#: MG203177

  • TrueORF®

Ccng1 (tGFP-tagged) - Mouse cyclin G1 (cDNA clone MGC:7684 IMAGE:3497034)

ORF Plasmid: DDK tGFP



  "BC005534" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,760.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Ccng, cyclin G
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG203177 representing BC005534
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGCAAGATTACGGGACTTTGAAGTAAAAGATCTACTTAGTCTAACTCAGTTCTTTGGCTTTGACA
CGGAGACATTTTCCCTAGCTGTGAATTTACTGGACAGATTCTTGTCTAAAATGAAGGTACAGGCGAAGCA
TCTTGGGTGTGTTGGACTGAGCTGCTTTTATTTGGCTGTGAAAGCGACTGAAGAGGAAAGGAATGTCCCA
CTGGCGACTGATTTGATCCGAATAAGTCAGTATAGGTTCACGGTTTCAGACCTGATGAGAATGGAGAAGA
TTGTGTTGGAGAAAGTGTGTTGGAAAGTCAAAGCTACTACTGCCTTTCAATTTCTGCAGCTCTATTATTC
ACTCGTTCACGACACCTTGCCATTTGAGAGGAGAAACGATCTGAATTTTGAAAGACTAGAAGCCCAACTT
AAGGCCTGCCACTGCAGGATCATATTTTCTAAGGCAAAGCCTTCTGTGCTGGCGCTATCTATCCTTGCGT
TGGAGATCCAAGCACTGAAATACGTAGAGTTAACAGAAGGAGTAGAATGTATTCAGAAACATTCCAAGAT
AAGTGGCCGAGATTTGACCTTCTGGCAAGAGCTTGTTTCCAAGTGTTTAACTGAATATTCATCAAACAAG
TGCTCCAAACCTAACGGTCAGAAGTTGAAATGGATTGTGTCTGGACGCACTGCACGGCAACTGAAGCACA
GTTATTATAGAATAACTCACCTCCCAACGATTCCTGAGACCATTTGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG203177 representing BC005534
Red=Cloning site Green=Tags(s)

MTARLRDFEVKDLLSLTQFFGFDTETFSLAVNLLDRFLSKMKVQAKHLGCVGLSCFYLAVKATEEERNVP
LATDLIRISQYRFTVSDLMRMEKIVLEKVCWKVKATTAFQFLQLYYSLVHDTLPFERRNDLNFERLEAQL
KACHCRIIFSKAKPSVLALSILALEIQALKYVELTEGVECIQKHSKISGRDLTFWQELVSKCLTEYSSNK
CSKPNGQKLKWIVSGRTARQLKHSYYRITHLPTIPETIC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC005534
ORF Size 747 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC005534, AAH05534
RefSeq Size 3151 bp
RefSeq ORF 749 bp
Locus ID 12450
Gene Summary May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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