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Taf9b (BC066223) Mouse Tagged ORF Clone
CAT＃: MG203166
- TrueORF®
Taf9b (tGFP-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (cDNA clone MGC:76785
ORF Plasmid: DDK
"BC066223" in other vectors (3)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BC066223; Taf9l |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203166 representing BC066223
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGGCCAAGATGGCGCCTATCAAGAACGCTCCGAGAGATGCCTTGGTGATGGCACAGATCCTGA AGGATATGGGAACCACAGAGTATGAGCCAAGGGTTATAAATCAAATGTTGGAATTTGCTTTCCGATATGT GACTACAATTCTGGACGATGCAAAGATTTATTCGAGTCATGCTAAGAAACCCACTGTTGATGCCGATGAC GTGAGACTGGCCATCCAGTGTCGGGCTGACCAGTCCTTTACCTCTCCTCCCCCAAGAGATTTTTTACTAG ATATTGCAAGGCAGAAGAATCAAACCCCTTTGCCGCTGATTAAGCCATATGCAGGACCCAGACTGCCACC TGATAGATACTGCTTAACAGCTCCAAACTATAGGCTGAAGTCCTTAGTTAAAAAGGGACCTAACCAAGGA AGACTAGTGCCTAGATTAAGTGCGGTCAGTAGTAGACCAACTACTCCTCCTGTAGCACCCCCACAAGCAG TATCTGGCCCAAATAAGGCTGCAACTCCGGTGTCAGTGACAAGCCAAAGATTTGCTGTGCAGATTCCACC TTCTCAGTCCACACCTGCCAAGCCAGCTCCTGCAGCAACTGCAGTCCAAAATGTTCTGATTAATCCTTCA ATGATTGGGCCCAAAAATATTCTCATTACCACCAGCATGGTTTCCTCACAGAACACAGCCACCGACTCAA ACCCACTGAAGAGGAAGCATGATGACGACGATGACAATGACACCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203166 representing BC066223
Red=Cloning site Green=Tags(s) MEPAKMAPIKNAPRDALVMAQILKDMGTTEYEPRVINQMLEFAFRYVTTILDDAKIYSSHAKKPTVDADD VRLAIQCRADQSFTSPPPRDFLLDIARQKNQTPLPLIKPYAGPRLPPDRYCLTAPNYRLKSLVKKGPNQG RLVPRLSAVSSRPTTPPVAPPQAVSGPNKAATPVSVTSQRFAVQIPPSQSTPAKPAPAATAVQNVLINPS MIGPKNILITTSMVSSQNTATDSNPLKRKHDDDDDNDTM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC066223 |
| ORF Size | 747 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC066223, AAH66223 |
| RefSeq Size | 2393 bp |
| RefSeq ORF | 749 bp |
| Locus ID | 407786 |
| Gene Summary | Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR203166 | Taf9b (Myc-DDK-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (cDNA clone MGC:76785 |
CNY 2,400.00 |
|
| MR203166L3 | Lenti ORF clone of Taf9b (Myc-DDK-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (cDNA clone MGC:76785 |
CNY 4,750.00 |
|
| MR203166L4 | Lenti ORF clone of Taf9b (mGFP-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (cDNA clone MGC:76785 |
CNY 4,750.00 |
