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Mrps27 (BC049149) Mouse Tagged ORF Clone
CAT＃: MG203136
- TrueORF®
Mrps27 (tGFP-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524)
ORF Plasmid: DDK
"BC049149" in other vectors (4)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610028H14Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203136 representing BC049149
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCAAGAAGCCTTTGACGTGCCGTCCACCCAGTTTCTCTCCCTCTATGTTTTATACCGTTGCCTAG CAGAGAAGACGGAGCTCACATGGGAAGAAGAAAGAGACTTTGGGGCGTCCCTCTTGCTTTCTGGCCTGAA ACAAAGGAACACAGTGGGCCTGAGTTCCCAGCTGTATGGCTATGCACTTCTCGGGAAGGTGGAACTGCAG CGTGGGGTGCGGGCTGTATACCACGGCATGCCTCTGATGTGGACACCAGGCTACCTCGACAGAGCCCTCC AAGTGATGGAGAGGGTGGCCTCCTCCCCTGAAGACCTCAAGCTGGGTAGAGAAGTGCTCGATGTGCTGGA TGGAGTCCTGAAGGTGGTGACGTCTCCAGATGTCCAGACTTCAGAGGCACAGCCTCAGGAAGGTGAAGAT GGCTTGGGCTCAGCAAACCTGGTGGAGCAGCTAGACACTGAGGAACCAGAACAGTCCAAGCTTCCCCGAT ACCTCGAGCGCTTTCAGGCCTCTCGGTCTAAACTGCAGGAGCTGAACAGAGTTGAGTCTGAAAGTCTTTT GACTCTGACCACCCAGCTTGTAAAAGAAAAACTCCCCGCCTGTGAGGCCGAGGACCTGGCCACCTACGAG CAGAAACTCCGTGAGTGGCACCTGGAGCGGGTGCAGTTGATCCAGAGGGAGCAGGAACAGAGGGAGAAGG CCAAGCAAGAGTACCAGGCGCTGAGTGCGGCAGAGAAAGCAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203136 representing BC049149
Red=Cloning site Green=Tags(s) MMQEAFDVPSTQFLSLYVLYRCLAEKTELTWEEERDFGASLLLSGLKQRNTVGLSSQLYGYALLGKVELQ RGVRAVYHGMPLMWTPGYLDRALQVMERVASSPEDLKLGREVLDVLDGVLKVVTSPDVQTSEAQPQEGED GLGSANLVEQLDTEEPEQSKLPRYLERFQASRSKLQELNRVESESLLTLTTQLVKEKLPACEAEDLATYE QKLREWHLERVQLIQREQEQREKAKQEYQALSAAEKAA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC049149 |
| ORF Size | 746 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC049149, AAH49149 |
| RefSeq Size | 1833 bp |
| RefSeq ORF | 746 bp |
| Locus ID | 218506 |
| Gene Summary | RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis. Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain. Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu). Overexpressed in hepatocellular carcinoma tissues compared with adjacent non-tumoral liver tissues.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217989 | Mrps27 (untagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524), (10ug) |
CNY 6,940.00 |
|
| MR203136 | Mrps27 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524) |
CNY 2,400.00 |
|
| MR203136L3 | Lenti ORF clone of Mrps27 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524) |
CNY 4,750.00 |
|
| MR203136L4 | Lenti ORF clone of Mrps27 (mGFP-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524) |
CNY 4,750.00 |
