Tfpi (BC036146) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	EPI, LACI
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG202854 representing BC036146 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTACAAAATGAAGAAAGAACATGCCTTTTGGGCCACTGTGTGTCTGTTGCTTAGCCTTGTTCCCG AGTTTCTTAATGCTCTGTCTGAGGAAGCTGATGACACAGATTCTGAGCTGGGGTCAATGAAACCGCTACA TACATTTTGTGCAATGAAGGCAGATGATGGTCCATGCAAAGCAATGATAAGGAGTTATTTTTTCAATATG TATACTCATCAATGTGAAGAATTTATATACGGGGGATGTGAAGGGAACGAGAACCGATTTGATACCCTGG AAGAGTGTAAGAAAACATGCATACCAGGTTATGAGAAGACAGCTGTGAAGGCAGCATCTGGAGCAGAAAG GCCAGATTTCTGCTTCTTGGAAGAGGACCCGGGACTCTGCCGAGGTTACATGAAGAGGTATCTTTATAAC AACCAGACAAAGCAGTGTGAACGATTCGTGTACGGTGGCTGCCTGGGCAACCGCAACAACTTTGAAACTT TGGATGAGTGCAAGAAGATCTGTGAGAATCCAGTCCACTCCCCTTCCCCAGTGAATGAGGTACAGATGAG TGACTACGTAACTGATGGAAATACTGTAACTGATCGCAGTACTGTAAATAACATCGTGGTTCCCCAGTCT CCCAAAGTGCCCAGGCGTCGGGCTGCCTTGGTACTGTATCACCTTTATGATATTTTGAAAAAACTCATCT TTGGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG202854 representing BC036146 Red=Cloning site Green=Tags(s) MTYKMKKEHAFWATVCLLLSLVPEFLNALSEEADDTDSELGSMKPLHTFCAMKADDGPCKAMIRSYFFNM YTHQCEEFIYGGCEGNENRFDTLEECKKTCIPGYEKTAVKAASGAERPDFCFLEEDPGLCRGYMKRYLYN NQTKQCERFVYGGCLGNRNNFETLDECKKICENPVHSPSPVNEVQMSDYVTDGNTVTDRSTVNNIVVPQS PKVPRRRAALVLYHLYDILKKLIFG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC036146
ORF Size	707 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC036146, AAH36146
RefSeq Size	3065 bp
RefSeq ORF	707 bp
Locus ID	21788
Gene Summary	Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma (By similarity).[UniProtKB/Swiss-Prot Function]

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