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Hsd17b2 (BC012682) Mouse Tagged ORF Clone
CAT＃: MG202707
- TrueORF®
Hsd17b2 (tGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 2 (cDNA clone MGC:13900 IMAGE:4217153)
ORF Plasmid: DDK
"BC012682" in other vectors (4)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI194836; AI194967; AI255511 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202707 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCGTTTGCCTCTGAGTCAGCATGGCTCTGCTTGGCTGCCGCTGCAGTGCTGGGGGGCACGCTTC TCTGCGGATGCCGGAGTGGCAGGCAGCTGCGGAGCCAGGCGGTGTGTCTCGCTGGCCTCTGGGGAGGTGC CTGCCTGCTCAGTCTGTCCCTCCTCTGCACCTTGTTCCTCTTATCCGTGGCCTGCTTCCTTCTTCTGTAT ATGAGTTCATCTGACCAAGACTTGTTACCTGTGGATCAAAAGGCCGTGCTGGTGACAGGTGCTGATTCCG GTTTCGGTCACGGTTTGGCCAAGCACCTGGACAAGCTGGGTTTCACAGTGTTTGCTGGAGTGCTGGACAA AGAGGGCCCGGGAGCAGAGGAACTGAGAAAACACTGCTCTGAGCGTCTTTCAGTGCTCCAGATGGATGTC ACCAAGCCAGAGCAGATAAAAGATGCTCACAGCAAAGTCACAGAGAAGATACAAGACAAAGGACTGTGGG CCGTGGTTAACAATGCCGGGGTCTTCCACTTACCTATCGACGGGGAACTCATACCCATGAGCATCTACAG AAAATGCATGGCCGTGAACTTCTTCGGAACTGTCGAGGTCACCAAGGCGTTTCTGCCTCTACTTAGAAAG TCCAAGGGGAGGCTGGTGAACGTCAGCAGCATGGGAGGCACGGTTCCACTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202707 protein sequence
Red=Cloning site Green=Tags(s) MSPFASESAWLCLAAAAVLGGTLLCGCRSGRQLRSQAVCLAGLWGGACLLSLSLLCTLFLLSVACFLLLY MSSSDQDLLPVDQKAVLVTGADSGFGHGLAKHLDKLGFTVFAGVLDKEGPGAEELRKHCSERLSVLQMDV TKPEQIKDAHSKVTEKIQDKGLWAVVNNAGVFHLPIDGELIPMSIYRKCMAVNFFGTVEVTKAFLPLLRK SKGRLVNVSSMGGTVPL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC012682 |
| ORF Size | 681 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC012682.1 |
| RefSeq Size | 1282 bp |
| RefSeq ORF | 684 bp |
| Locus ID | 15486 |
| MW | 24.2 kDa |
| Gene Summary | Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200820 | Hsd17b2 (untagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 2 (cDNA clone MGC:13900 IMAGE:4217153), (10ug) |
CNY 2,400.00 |
|
| MR202707 | Hsd17b2 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 2 (Hsd17b2) |
CNY 3,656.00 |
|
| MR202707L3 | Lenti ORF clone of Hsd17b2 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 2 (Hsd17b2) |
CNY 4,750.00 |
|
| MR202707L4 | Lenti ORF clone of Hsd17b2 (mGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 2 (Hsd17b2) |
CNY 4,750.00 |
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