Chchd3 (BC021941) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	0610041L09Rik; 1700039J09Rik; AW558177
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG202692 representing BC021941 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGGGACCGCTAGCACCCGCCGGGTCACCTTCGAGGCGGACGAGAACGAGAACATCACCGTGGTGA AGGGCATCCGGCTTTCAGAAAATGTGATCGACCGGATGAAGGAGTCCTCTCCATCTGGCTCTAAGTCTCA GCGATATTCCAGCGTTTATGGTGCTTCAGTTTCTGATGAAGATTTGAAAAGAAGAGTAGCTGAGGAGCTG GCATTGGAGCAAGCCAAAAAGGAGTCAGAACACCAGAGACGCCTTAAGCAAGCAAGGGACCTAGAAAGAG AAAGGGCTGCAGCCAATGAGCAGCTGACCAGAGCTGTCCTTCGGGAAAGAATATCCAGTGAAGAGGAGCG CATGAAGGCGAAGCATCTGGCTCGGCAGCTGGAAGAGAAAGACCGAGTGATGCGGAAGCAGGATGCATTC TACAAAGAGCAGCTGGCCAGACTGGAGGAGAGGAGCTCAGAGTTCTACAAAGTCACCACCGAGGAGTATC AGAAAGCTGCTGAAGAGGTGGAAGCCAAGTTCAAGCGGTATGAATATCATCCAGTCTGTGCAGATCTGCA GACCAAAATCCTCCAGTGTTACCGTCAGAACACCCAGCAGACTCTCAGCTGCTCTGCTCTGGCTAGCCAA TACATGCACTGTGTCAACCATGCCAAACAGAGCATGCTGGAGAAGGGAGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG202692 representing BC021941 Red=Cloning site Green=Tags(s) MGGTASTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSSVYGASVSDEDLKRRVAEEL ALEQAKKESEHQRRLKQARDLERERAAANEQLTRAVLRERISSEEERMKAKHLARQLEEKDRVMRKQDAF YKEQLARLEERSSEFYKVTTEEYQKAAEEVEAKFKRYEYHPVCADLQTKILQCYRQNTQQTLSCSALASQ YMHCVNHAKQSMLEKGG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC021941
ORF Size	683 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021941, AAH21941
RefSeq Size	1084 bp
RefSeq ORF	683 bp
Locus ID	66075
Gene Summary	Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology.[UniProtKB/Swiss-Prot Function]

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