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Spc25 (BC027121) Mouse Tagged ORF Clone
CAT＃: MG202652
- TrueORF®
Spc25 (tGFP-tagged) - Mouse SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) (cDNA clone MGC:38808 IMAGE:5359960)
ORF Plasmid: DDK
"BC027121" in other vectors (4)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2600017H08Rik; 2610205L13Rik; Spbc25 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202652 representing BC027121
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGAAGACGAATTGGCACTCTTAAATCAAAGCATCAATGAATTTGGGGATAAGTTCAGAAACAGAC TCGATGATAATCATAGTCAAGTGCTGGGACTTAGAGACGCCTTCAAGGACTCCATGAAAGCGTTTTCAGA AAAGATGTCTTTGAAATTAAAGGAAGAAGAGAGGATGACTGAGATGATTCTGGAGTATAAAAACCAGCTC TGTAAGCAGAATAAGCTCATTCAAGAAAAGAAAGAGAATGTGTTGAAGATGATTGCTGAAGTAAAAGGCA AGGAGCAAGAGTCGGAAGAGCTGACTGCTAAAATCCAGGAGCTCAAGGAAGAGTACGCTAGGAAGAGGGA AACCATTTCCACTGCTAACAAAGCTAATGAAGAGAGATTGAAAGGACTGCAGAAATCAGCGGATCTGTAT AGAGATTACCTTGGACTAGAAATTAGAAAGATTCACGGTAATAAATTGCAGTTTATATTTACTAGTATTG ACCCTAAGAATCCTGAGAGCCCATATATGTTTTCCATGAGCATAAATGAAGCTAAGGAATATGAAGTGTA CGACAGTTCGCCTCATCTTGAGTGCCTAGCAGAATTTCAGGAGAAAGTCAGGAAGACCAACAATTTTTCA GCTTTTCTTGCCAATATTCGGAAGGCTTTTATAGCTAAGGTTCATAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202652 representing BC027121
Red=Cloning site Green=Tags(s) MGEDELALLNQSINEFGDKFRNRLDDNHSQVLGLRDAFKDSMKAFSEKMSLKLKEEERMTEMILEYKNQL CKQNKLIQEKKENVLKMIAEVKGKEQESEELTAKIQELKEEYARKRETISTANKANEERLKGLQKSADLY RDYLGLEIRKIHGNKLQFIFTSIDPKNPESPYMFSMSINEAKEYEVYDSSPHLECLAEFQEKVRKTNNFS AFLANIRKAFIAKVHN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC027121 |
| ORF Size | 680 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027121, AAH27121 |
| RefSeq Size | 1344 bp |
| RefSeq ORF | 680 bp |
| Locus ID | 66442 |
| Gene Summary | This gene encodes a component of the kinetochore-associated NDC80 protein complex, which is required for the mitotic spindle checkpoint and for microtubule-kinetochore attachment. During meiosis in mouse, the protein localizes to the germinal vesicle and then is associated with the chromosomes following germinal vesicle breakdown. Knockdown of this gene in oocytes results in precocious polar body extrusion, chromosome misalignment and aberrant spindle formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217821 | Spc25 (untagged) - Mouse SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) (cDNA clone MGC:38808 IMAGE:5359960),, (10ug) |
CNY 6,940.00 |
|
| MR202652 | Spc25 (Myc-DDK-tagged) - Mouse SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) (cDNA clone MGC:38808 IMAGE:5359960) |
CNY 2,400.00 |
|
| MR202652L3 | Lenti ORF clone of Spc25 (Myc-DDK-tagged) - Mouse SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) (cDNA clone MGC:38808 IMAGE:5359960) |
CNY 4,750.00 |
|
| MR202652L4 | Lenti ORF clone of Spc25 (mGFP-tagged) - Mouse SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) (cDNA clone MGC:38808 IMAGE:5359960) |
CNY 4,750.00 |
