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Hes6 (NM_019479) Mouse Tagged ORF Clone
CAT＃: MG202615
- TrueORF®
Hes6 (tGFP-tagged) - Mouse hairy and enhancer of split 6 (Drosophila) (Hes6)
ORF Plasmid: DDK
"NM_019479" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI326893; bHLHb41 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202615 representing NM_019479
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCGTCCCAGGCGCCCAGCCGGGACCGTGCAGGCCAGGAGGATGAGGACCGCTGGGAAGCACGGG GGGACCGCAAGGCCCGGAAGCCCTTGGTGGAGAAGAAGCGACGCGCACGGATCAACGAGAGTCTTCAGGA GCTGCGGCTGCTGCTGGCCGGTACCGAGGTGCAGGCCAAGCTAGAGAACGCCGAGGTTCTGGAGCTGACC GTGAGGCGCGTGCAGGGCGCGCTGCGAGGCCGGGCGCGCGAGCGGGAGCAGCTGCAGGCTGAAGCAAGCG AGCGCTTCGCTGCTGGCTACATCCAGTGCATGCATGAGGTGCACACGTTCGTGTCCACGTGCCAAGCCAT CGATGCCACTGTCTCAGCTGAACTCCTGAACCACCTGCTAGAATCCATGCCACTGCGCGAGGGTAGCAGC TTTCAGGATCTGCTGGGGGACTCCCTAGCTGGGCTGCCTGGAGGCTCTGGGAGGAGCAGCTGGCCTCCAG GAGGGTCCCCAGAATCCCCATTGTCCAGTCCCCCAGGTCCCGGGGACGACCTGTGTTCTGACCTAGAGGA GATCCCAGAGGCTGAACTAAACCGGGTGCCTGCTGAGGGGCCGGATTTGGTGTCTACATCCTTAGGCAGC CTGACTGCAGCTCGCCGGGCTCAGAGTGTGTGGAGGCCTTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202615 representing NM_019479
Red=Cloning site Green=Tags(s) MAPSQAPSRDRAGQEDEDRWEARGDRKARKPLVEKKRRARINESLQELRLLLAGTEVQAKLENAEVLELT VRRVQGALRGRAREREQLQAEASERFAAGYIQCMHEVHTFVSTCQAIDATVSAELLNHLLESMPLREGSS FQDLLGDSLAGLPGGSGRSSWPPGGSPESPLSSPPGPGDDLCSDLEEIPEAELNRVPAEGPDLVSTSLGS LTAARRAQSVWRPW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019479 |
| ORF Size | 672 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019479.2, NP_062352.1 |
| RefSeq Size | 1336 bp |
| RefSeq ORF | 675 bp |
| Locus ID | 55927 |
| UniProt ID | Q9JHE6 |
| Gene Summary | Does not bind DNA itself but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207510 | Hes6 (untagged) - Mouse hairy and enhancer of split 6 (Drosophila) (Hes6), (10ug) |
CNY 2,400.00 |
|
| MR202615 | Hes6 (Myc-DDK-tagged) - Mouse hairy and enhancer of split 6 (Drosophila) (Hes6) |
CNY 2,400.00 |
|
| MR202615L3 | Lenti ORF clone of Hes6 (Myc-DDK-tagged) - Mouse hairy and enhancer of split 6 (Drosophila) (Hes6) |
CNY 4,750.00 |
|
| MR202615L4 | Lenti ORF clone of Hes6 (mGFP-tagged) - Mouse hairy and enhancer of split 6 (Drosophila) (Hes6) |
CNY 4,750.00 |
