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Efna1 (NM_010107) Mouse Tagged ORF Clone
CAT＃: MG202142
- TrueORF®
Efna1 (tGFP-tagged) - Mouse ephrin A1 (Efna1)
"NM_010107" in other vectors (5)
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CNY 4,000.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI325262; B61; Efl1; Epl1; Eplg1; Lerk1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202142 representing NM_010107
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCTTTGGGCCCCTCTCTTGGGTCTGTGCTGCAGTCTGGCCGCTGCTGACCGCCACATCGTCT TCTGGAACAGTTCAAATCCCAAGTTCCGTGAGGAGGACTACACGGTGCACGTGCAGCTGAATGACTACCT AGACATCATCTGCCCACATTACGAGGACGACTCTGTGGCAGATGCAGCCATGGAGCGATACACACTGTAC ATGGTGGAACACCAGGAGTATGTGGCATGCCAACCCCAGTCCAAGGACCAGGTCCGTTGGAATTGCAACC GGCCCAGTGCCAAGCATGGCCCGGAGAAGCTGTCTGAGAAATTCCAGCGCTTCACGCCTTTTATCTTGGG CAAGGAGTTCAAGGAAGGACACAGCTACTACTACATCTCCAAACCTATCTACCATCAGGAATCCCAGTGC TTGAAGCTGAAGGTGACTGTCAATGGCAAAATCACTCATAATCCCCAGGCCCATGTCAACCCACAGGAGA AGAGACTCCAAGCAGATGACCCGGAAGTACAGGTTTTGCACAGCATTGGTTACAGTGCCGCCCCCCGCCT CTTCCCACTGGTCTGGGCAGTATTGCTCCTACCACTGCTGCTGCTGCAATCTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202142 representing NM_010107
Red=Cloning site Green=Tags(s) MEFLWAPLLGLCCSLAAADRHIVFWNSSNPKFREEDYTVHVQLNDYLDIICPHYEDDSVADAAMERYTLY MVEHQEYVACQPQSKDQVRWNCNRPSAKHGPEKLSEKFQRFTPFILGKEFKEGHSYYYISKPIYHQESQC LKLKVTVNGKITHNPQAHVNPQEKRLQADDPEVQVLHSIGYSAAPRLFPLVWAVLLLPLLLLQSQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010107 |
| ORF Size | 615 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010107.4, NP_034237.3 |
| RefSeq Size | 1474 bp |
| RefSeq ORF | 618 bp |
| Locus ID | 13636 |
| UniProt ID | P52793 |
| Gene Summary | Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200310 | Efna1 (untagged) - Mouse ephrin A1 (Efna1), transcript variant 1, (10ug) |
CNY 2,400.00 |
|
| MG226172 | Efna1 (tGFP-tagged) - Mouse ephrin A1 (Efna1) transcript variant 1, (10ug) |
CNY 2,280.00 |
|
| MR226172 | Efna1 (Myc-DDK-tagged) - Mouse ephrin A1 (Efna1), transcript variant 1 |
CNY 2,090.00 |
|
| MR226172L3 | Lenti ORF clone of Efna1 (Myc-DDK-tagged) - Mouse ephrin A1 (Efna1), transcript variant 1 |
CNY 3,990.00 |
|
| MR226172L4 | Lenti ORF clone of Efna1 (mGFP-tagged) - Mouse ephrin A1 (Efna1), transcript variant 1 |
CNY 3,990.00 |
