Wibg (BC049647) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	A030010B05Rik; Pym; Wibg
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG202089 representing BC049647 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACTCCCTATGTAACTGACGAGACCGGCGGCAAGTACATTGCCTCAACACAGAGACCCGACGGGA CGTGGCGAAAGCAGCGGAGGGTCAAAGAAGGATACGTGCCCCAAGAGGAGGTCCCAGTCTATGAAAACAA GTATGTGAAGTTTTTCAAGAGTAAACCAGAACTTCCCCCAGGGCTGAGCCCTGAGGCCACCACACCGGTC ACCCCATCCAGACCTGAAGGTGGTGAGACAGGCCTCTCCAAGACAGCCAAACGAAACCTGAAGCGGAAGG AGAAGAGGCGGCAGCAACAGGAGAAAGAAGCAGAGGCCTTGAGCAGGACTCTTGATAAGGTGTCTCTGGG AGACACAGCCCAGATACCTAGTGCTCTCCAAGGCCCCCAGGCCACGCCTCTAGCTGCCTCTGATCCATCC GACTCTGCTGCCACCACAGAGAAAGCCAAGAAGATCAAGAACCTGAGGAAGAAGCTTCGGCAGGTGGAGG AGCTGCAGCAACGCATCCAGGCCGGGGAGGTCAGCCAGCCCAGCAGAGAACAGCTGGAGAAGCTGGCTCG ACGGAGGGTTCTGGAGGAGGAGCTGGAGGACTTGGAGTTGGGCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG202089 representing BC049647 Red=Cloning site Green=Tags(s) MATPYVTDETGGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYVKFFKSKPELPPGLSPEATTPV TPSRPEGGETGLSKTAKRNLKRKEKRRQQQEKEAEALSRTLDKVSLGDTAQIPSALQGPQATPLAASDPS DSAATTEKAKKIKNLRKKLRQVEELQQRIQAGEVSQPSREQLEKLARRRVLEEELEDLELGL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC049647
ORF Size	608 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC049647, AAH49647
RefSeq Size	1120 bp
RefSeq ORF	608 bp
Locus ID	78428
Gene Summary	Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmark for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as an EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions (By similarity).[UniProtKB/Swiss-Prot Function]

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