Cryba4 (NM_021351) Mouse Tagged ORF Clone
CAT#: MG201946
- TrueORF®
Cryba4 (tGFP-tagged) - Mouse crystallin, beta A4 (Cryba4)
ORF Plasmid: DDK
"NM_021351" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201946 representing NM_021351
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGCAGTGCACCAAGTCAGCTGGACACTGGAGGATGGTGGTGTGGGATGAAGAAGGCTTCCAGG GCCGACGGCATGAATTCACAGCTGAGTGTCCCAGTGTCCTGGAACTTGGTTTTGAGACGGTGCGATCTCT CAAAGTCCTGAGCGGAGCGTGGGTAGGCTTTGAGCACGCCGGCTTCCAAGGACAGCAATATGTGCTGGAG AGGGGCGATTACCCGGGCTGGGATGCCTGGGGTGGCAACACAGCCTACCCTGCGGAGAGGCTCACCTCCT TCCGGCCTGTGGCCTGCGCTAACCACCGCGACTCAAGGCTGACCATCTTCGAGCAGGAGAACTTCCTGGG CAGGAAAGGCGAGCTGAACGATGACTATCCCTCTCTGCAGGCCATGGGCTGGGACGGCACTGAAGTGGGC TCCTTCCATGTTCAATCTGGGGCGTGGGTTTGTTCCCAGTTTCCTGGCTACCGAGGTTTTCAGTACATCC TGGAGAGCGATCACCACTCAGGTGACTACAAGCACTTCAGAGAGTGGGGCTCCCATGCTCACACCTTCCA GGTGCAGAGTGTGCGCAGAATCCAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201946 representing NM_021351
Red=Cloning site Green=Tags(s) MTLQCTKSAGHWRMVVWDEEGFQGRRHEFTAECPSVLELGFETVRSLKVLSGAWVGFEHAGFQGQQYVLE RGDYPGWDAWGGNTAYPAERLTSFRPVACANHRDSRLTIFEQENFLGRKGELNDDYPSLQAMGWDGTEVG SFHVQSGAWVCSQFPGYRGFQYILESDHHSGDYKHFREWGSHAHTFQVQSVRRIQQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021351 |
ORF Size | 588 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021351.2 |
RefSeq Size | 752 bp |
RefSeq ORF | 591 bp |
Locus ID | 12959 |
UniProt ID | Q9JJV0 |
Gene Summary | This gene encodes a member of the crystallin family of proteins that contribute to the transparency and refractive properties of the ocular lens. Certain mutations in the human ortholog of this gene are associated with cataract and bilateral microphthalmia. This gene is located adjacent to a related crystallin gene on chromosome 5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202680 | Cryba4 (untagged) - Mouse crystallin, beta A4 (Cryba4), (10ug) |
CNY 2,400.00 |
|
MR201946 | Cryba4 (Myc-DDK-tagged) - Mouse crystallin, beta A4 (Cryba4) |
CNY 2,400.00 |
|
MR201946L3 | Lenti ORF clone of Cryba4 (Myc-DDK-tagged) - Mouse crystallin, beta A4 (Cryba4) |
CNY 4,750.00 |
|
MR201946L4 | Lenti ORF clone of Cryba4 (mGFP-tagged) - Mouse crystallin, beta A4 (Cryba4) |
CNY 4,750.00 |