Dhps (BC039963) Mouse Tagged ORF Clone
CAT#: MG201742
- TrueORF®
Dhps (tGFP-tagged) - Mouse deoxyhypusine synthase (cDNA clone MGC:49129 IMAGE:4240646)
ORF Plasmid: DDK
"BC039963" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Dhs, MGC49129, MGC74384 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201742 representing BC039963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCATTCTGGACCAGATGGTGCTGGAGCAGAACACAGAGGGTGTGAAGTGGACACCTTCCAAGATGA TCTCCCGGCTTGGGAAGGAGATCAACAACCCAGACTCTGTGTATTATTGGGCCCATAAGAACCACATCCC TGTGCTGAGTCCAGCACTCACGGATGGCTCACTGGGTGACATGATCTTCTTCCATTCCTATAAAAACCCA GGCTTGGTCCTGGACATTGTTGAAGACCTGCGACTCATCAACACGCAGGCCATTTTCGCCAAGCGCTCTG GGATGATCATCCTGGGTGGAGGTGTGGTCAAGCACCACATTGCCAACGCTAACCTCATGAGGAATGGAGC AGACTACGCTGTTTATATCAACACAGCCCAGGAGTTTGATGGCTCAGACTCCGGAGCCCGGCCAGATGAG GCTGTCTCTTGGGGCAAGATCCGGATGGACGCACAGCCAGTAAAGGTCTATGCTGATGCTTCTCTGGTTT TCCCCTTGCTGGTGGCTGAGACATTCGCCCAAAAGGCAGATGCCTTCAGAGCTGAGAAGAATGAAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201742 representing BC039963
Red=Cloning site Green=Tags(s) MPILDQMVLEQNTEGVKWTPSKMISRLGKEINNPDSVYYWAHKNHIPVLSPALTDGSLGDMIFFHSYKNP GLVLDIVEDLRLINTQAIFAKRSGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDE AVSWGKIRMDAQPVKVYADASLVFPLLVAETFAQKADAFRAEKNED TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC039963 |
ORF Size | 560 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC039963, AAH39963 |
RefSeq Size | 1149 bp |
RefSeq ORF | 560 bp |
Locus ID | 330817 |
Gene Summary | Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC206999 | Dhps (untagged) - Mouse deoxyhypusine synthase (cDNA clone MGC:49129 IMAGE:4240646), (10ug) |
CNY 3,230.00 |
|
MR201742 | Dhps (Myc-DDK-tagged) - Mouse deoxyhypusine synthase (cDNA clone MGC:49129 IMAGE:4240646) |
CNY 2,400.00 |
|
MR201742L3 | Lenti ORF clone of Dhps (Myc-DDK-tagged) - Mouse deoxyhypusine synthase (cDNA clone MGC:49129 IMAGE:4240646) |
CNY 4,750.00 |
|
MR201742L4 | Lenti ORF clone of Dhps (mGFP-tagged) - Mouse deoxyhypusine synthase (cDNA clone MGC:49129 IMAGE:4240646) |
CNY 4,750.00 |