Ift27 (NM_025931) Mouse Tagged ORF Clone
CAT#: MG201739
- TrueORF®
Ift27 (tGFP-tagged) - Mouse RAB, member of RAS oncogene family-like 4 (Rabl4)
ORF Plasmid: DDK
"NM_025931" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2600013G09Rik; Rabl4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201739 representing NM_025931
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAAGCTAGCTGCCAAATGCATCCTGGCAGGGGACCCAGCCGTAGGCAAGACGGCCCTGGTGCAGA TGTTCCGCAGCGACGGGACCCATTTCCAGAAGAACTACACCCTGACAACTGGAGTGGATTTGGTGGTGAA GACAGTGCCAGTTCTTGACACAAATGACAGTGTGGAACTCTTCATTTTTGACTCAGCCGGCAAGGAGCTG TTCTCTGAAATGCTGGATAAGTTGTGGGAGAATCCCAACGTCCTGTGCCTTGTCTATGATGTGACCAATG AACAGTCCTTCATCAGCTGCACCAAGTGGTTGGAGAAGGTCCGGTCTCAGACTTCAGGCATCTCCCTCCC AGGTGTTTTAGTGGGAACTAAGACAGACCTGGCTGGCCGACAAACAGTGGATTCAGCTCAGGCCCAGGTA TGGGCACTGAGTCAAGGCCTGGAATTCTTTGAGACATCTGTGAAGGAGATGGATAATTACGAGGCCCCGT TCCACTGTCTGGCCAAGCAGTTCTACCAGCTGTACCGGGAAAAAGTGGACATATTCCATACCCTGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201739 representing NM_025931
Red=Cloning site Green=Tags(s) MVKLAAKCILAGDPAVGKTALVQMFRSDGTHFQKNYTLTTGVDLVVKTVPVLDTNDSVELFIFDSAGKEL FSEMLDKLWENPNVLCLVYDVTNEQSFISCTKWLEKVRSQTSGISLPGVLVGTKTDLAGRQTVDSAQAQV WALSQGLEFFETSVKEMDNYEAPFHCLAKQFYQLYREKVDIFHTLV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025931 |
ORF Size | 558 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025931.3 |
RefSeq Size | 1050 bp |
RefSeq ORF | 561 bp |
Locus ID | 67042 |
UniProt ID | Q9D0P8 |
Gene Summary | Small GTPase-like component of the intraflagellar transport (IFT) complex B that promotes the exit of the BBSome complex from cilia via its interaction with ARL6 (PubMed:25446516). Not involved in entry of the BBSome complex into cilium. Prevents aggregation of GTP-free ARL6. Required for hedgehog signaling (PubMed:25446516). Forms a subcomplex within the IFT complex B with IFT25 (By similarity). Its role in intraflagellar transport is mainly seen in tissues rich in ciliated cells such as kidney and testis. Essential for male fertility, spermiogenesis and sperm flagella formation (PubMed:28964737). Plays a role in the early development of the kidney (PubMed:29626631). May be involved in the regulation of ureteric bud initiation (PubMed:29626631).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203941 | Ift27 (untagged) - Mouse intraflagellar transport 27 homolog (Chlamydomonas) (Ift27), (10ug) |
CNY 2,400.00 |
|
MR201739 | Ift27 (Myc-DDK-tagged) - Mouse intraflagellar transport 27 homolog (Chlamydomonas) (Ift27) |
CNY 2,400.00 |
|
MR201739L3 | Lenti ORF clone of Ift27 (Myc-DDK-tagged) - Mouse intraflagellar transport 27 homolog (Chlamydomonas) (Ift27) |
CNY 4,750.00 |
|
MR201739L4 | Lenti ORF clone of Ift27 (mGFP-tagged) - Mouse intraflagellar transport 27 homolog (Chlamydomonas) (Ift27) |
CNY 4,800.00 |