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Igf2 (BC053489) Mouse Tagged ORF Clone
CAT＃: MG201606
- TrueORF®
Igf2 (tGFP-tagged) - Mouse insulin-like growth factor 2 (cDNA clone MGC:60598 IMAGE:30013295)
ORF Plasmid: DDK
"BC053489" in other vectors (4)
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CNY 5,200.00
| Cited in 1 publication. |
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Igf-II, Mpr, M6pr, Peg2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201606 representing BC053489
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATCCCAGTGGGGAAGTCGATGTTGGTGCTTCTCATCTCTTTGGCCTTCGCCTTGTGCTGCATCG CTGCTTACGGCCCCGGAGAGACTCTGTGCGGAGGGGAGCTTGTTGACACGCTTCAGTTTGTCTGTTCGGA CCGCGGCTTCTACTTCAGCAGGCCTTCAAGCCGTGCCAACCGTCGCAGCCGTGGCATCGTGGAAGAGTGC TGCTTCCGCAGCTGCGACCTGGCCCTCCTGGAGACATACTGTGCCACCCCCGCCAAGTCCGAGAGGGACG TGTCTACCTCTCAGGCCGTACTTCCGGACGACTTCCCCAGATACCCCGTGGGCAAGTTCTTCCAATATGA CACCTGGAGACAGTCCGCGGGACGCCTGCGCAGAGGCCTGCCTGCCCTCCTGCGTGCCCGCCGGGGTCGC ATGCTTGCCAAAGAGCTCAAAGAGTTCAGAGAGGCCAAACGTCATCGTCCCCTGATCGTGTTACCACCCA AAGACCCCGCCCACGGGGGAGCCTCTTCGGAGATGTCCAGCAACCATCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201606 representing BC053489
Red=Cloning site Green=Tags(s) MGIPVGKSMLVLLISLAFALCCIAAYGPGETLCGGELVDTLQFVCSDRGFYFSRPSSRANRRSRGIVEEC CFRSCDLALLETYCATPAKSERDVSTSQAVLPDDFPRYPVGKFFQYDTWRQSAGRLRRGLPALLRARRGR MLAKELKEFREAKRHRPLIVLPPKDPAHGGASSEMSSNHQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC053489 |
| ORF Size | 540 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC053489, AAH53489 |
| RefSeq Size | 1532 bp |
| RefSeq ORF | 542 bp |
| Locus ID | 16002 |
| Gene Summary | This gene encodes a member of the insulin-like growth factor (IGF) family of proteins that promote growth and development during fetal and postnatal life. It is an imprinted gene that is expressed only from the paternal allele. The encoded protein undergoes proteolytic processing to generate a mature peptide. The transgenic overexpression of this gene in mice results in prenatal overgrowth, polyhydramnios, fetal and neonatal lethality, disproportionate organ overgrowth including tongue enlargement, and skeletal abnormalities. Mice lacking the encoded protein exhibit growth deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture
,null,
Nature medicine
,PubMed ID 24859528
[Igf2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206836 | Igf2 (untagged) - Mouse insulin-like growth factor 2 (cDNA clone MGC:60598 IMAGE:30013295), (10ug) |
CNY 3,600.00 |
|
| MR201606 | Igf2 (Myc-DDK-tagged) - Mouse insulin-like growth factor 2 (cDNA clone MGC:60598 IMAGE:30013295) |
CNY 3,600.00 |
|
| MR201606L3 | Lenti ORF clone of Igf2 (Myc-DDK-tagged) - Mouse insulin-like growth factor 2 (cDNA clone MGC:60598 IMAGE:30013295) |
CNY 5,890.00 |
|
| MR201606L4 | Lenti ORF clone of Igf2 (mGFP-tagged) - Mouse insulin-like growth factor 2 (cDNA clone MGC:60598 IMAGE:30013295) |
CNY 6,000.00 |
