Taf9 (BC019453) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2810046E22Rik; 4921516M08Rik; AK/ATPase
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG201449 representing BC019453 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTTCCGAATATCCTGCTCACCGGTACACCAGGGGTTGGAAAAACCACACTAGGCAAAGAACTTG CATCAAGATCAGGCCTGAAGTACGTTAACGTGGGAGATCTAGCTCGAGAAGGGCAGTTATACGACGGCTA CGATGAGGAGTATGGCTGTCCCATTCTAGATGAGGATAGGGTTGTCGATGAGTTAGAGCATCAGATGCAG GAGGGCGGTGTGATTGTGGATTACCACGGCTGTGACTTCTTCCCCGAACGCTGGTTTCATATAGTGTTTG TGTTGAGAACAGATAATGGTGTATTGTATAAACGACTTGAAACAAGGGGTTATAATGAGAAGAAACTACA AGACAATATTCAGTGTGAGATTTTTCAAGTTCTTTATGAAGAAGCCATAGCATCTTACAAAGAAGAAATC GTGCATCAGCTGCCCAGTAATGAACCAGAACAGCTCGAGGACAATATCAACCAAATCTCAAAATGGATTG AGCAGTGGGTCAAAGATCATAACCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG201449 representing BC019453 Red=Cloning site Green=Tags(s) MKLPNILLTGTPGVGKTTLGKELASRSGLKYVNVGDLAREGQLYDGYDEEYGCPILDEDRVVDELEHQMQ EGGVIVDYHGCDFFPERWFHIVFVLRTDNGVLYKRLETRGYNEKKLQDNIQCEIFQVLYEEAIASYKEEI VHQLPSNEPEQLEDNINQISKWIEQWVKDHNP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC019453
ORF Size	518 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019453, AAH19453
RefSeq Size	907 bp
RefSeq ORF	518 bp
Locus ID	108143
Gene Summary	Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function]

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