Btf3 (BC008233) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1700054E11Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG201278 representing BC008233 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGAAACAATCATGAACCAGGAGAAACTCGCCAAACTGCAGGCACAAGTGCGCATTGGTGGGAAAG GAACTGCTCGTAGGAAGAAGAAGGTGGTTCACAGAACGGCCACAGCAGACGATAAGAAACTGCAGTTCTC CTTAAAGAAGTTAGGGGTGAACAACATCTCTGGTATTGAAGAGGTGAACATGTTTACAAACCAAGGAACA GTGATCCATTTTAACAACCCTAAAGTTCAGGCATCCCTGGCAGCAAACACCTTCACCATTACAGGCCACG CTGAGACAAAGCAGCTGACAGAAATGCTTCCCAGCATCCTCAACCAGCTTGGTGCAGACAGCCTGACTAG TTTAAGGAGACTGGCTGAAGCTCTGCCCAAACAATCTGTGGATGGAAAAGCACCCCTTGCTACTGGAGAG GATGATGATGATGAAGTTCCAGATCTGGTGGAGAATTTTGATGAGGCTTCTAAGAATGAGGCAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG201278 representing BC008233 Red=Cloning site Green=Tags(s) MKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGT VIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGE DDDDEVPDLVENFDEASKNEAN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC008233
ORF Size	488 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC008233, AAH08233
RefSeq Size	864 bp
RefSeq ORF	488 bp
Locus ID	218490
Gene Summary	When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription (By similarity).[UniProtKB/Swiss-Prot Function]

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