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Hscb (NM_153571) Mouse Tagged ORF Clone
CAT＃: MG201219
- TrueORF®
Hscb (tGFP-tagged) - Mouse HscB iron-sulfur cluster co-chaperone homolog (E
ORF Plasmid: DDK
"NM_153571" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI325508; AW049829; Hsc20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201219 representing NM_153571
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTGCAACCGCTCCTTCAGGGTGGACGTTACGAAACTTCAGCACAGGTACCAGCAACTGCAGCGGC TTGTCCACCCAGATTTCTTCAGCCAAAAGTCTCAGACTGAAAAACACTTCTCTGACAAGCACTCCACCCT GGTGAATGATGCCTATAAGACTCTTCAGGCTCCCCTGACCAGAGGACTATATCTTCTAAAGCTCCAGGGA ATAGAAATTCCTGAAGGGACAGATTACAAAGCAGACAGTCAGTTCCTTGTGGAAATCATGGAAATCAATG AAAGACTCGCAGACGCCCAAAGTGAGGCCGCCATGGAAGAGATAGAAGCCACTGTCAGAGCTAAACAGAA AGAATTTACTGACAATATAAACAGCGCTTTTGAACAAGGTGACTTTGAAAAAGCCAAGGAACTCCTGACA AAGATGAGATACTTTTCGAACATAGAAGAAAAGATCAAGCTAAGCAAGACTCCTCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201219 representing NM_153571
Red=Cloning site Green=Tags(s) MNCNRSFRVDVTKLQHRYQQLQRLVHPDFFSQKSQTEKHFSDKHSTLVNDAYKTLQAPLTRGLYLLKLQG IEIPEGTDYKADSQFLVEIMEINERLADAQSEAAMEEIEATVRAKQKEFTDNINSAFEQGDFEKAKELLT KMRYFSNIEEKIKLSKTPL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153571 |
| ORF Size | 477 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153571.1, NP_705799.1 |
| RefSeq Size | 789 bp |
| RefSeq ORF | 705 bp |
| Locus ID | 100900 |
| UniProt ID | Q8K3A0 |
| Gene Summary | Acts as a co-chaperone in iron-sulfur cluster assembly in both mitochondria and the cytoplasm. Required for incorporation of iron-sulfur clusters into SDHB, the iron-sulfur protein subunit of succinate dehydrogenase that is involved in complex II of the mitochondrial electron transport chain. Recruited to SDHB by interaction with SDHAF1 which first binds SDHB and then recruits the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20. Also mediates complex formation between components of the cytosolic iron-sulfur biogenesis pathway and the CIA targeting complex composed of CIAO1, DIPK1B/FAM69B and MMS19 by binding directly to the scaffold protein ISCU and to CIAO1. This facilitates iron-sulfur cluster insertion into a number of cytoplasmic and nuclear proteins including POLD1, ELP3, DPYD and PPAT.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201343 | Hscb (untagged) - Mouse HscB iron-sulfur cluster co-chaperone homolog (E. coli) (Hscb), (10ug) |
CNY 1,200.00 |
|
| MR201219 | Hscb (Myc-DDK-tagged) - Mouse HscB iron-sulfur cluster co-chaperone homolog (E. coli) (Hscb) |
CNY 1,200.00 |
|
| MR201219L3 | Lenti ORF clone of Hscb (Myc-DDK-tagged) - Mouse HscB iron-sulfur cluster co-chaperone homolog (E. coli) (Hscb) |
CNY 4,750.00 |
|
| MR201219L4 | Lenti ORF clone of Hscb (mGFP-tagged) - Mouse HscB iron-sulfur cluster co-chaperone homolog (E. coli) (Hscb) |
CNY 4,750.00 |
